ArticleInfection and immunity2009
The purified and recombinant Legionella pneumophila chaperonin alters mitochondrial trafficking and microfilament organization.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- The Passage of Chaperonins to Extracellular Locations inBiomolecules · 2025Article
- The hidden dancers in water: the symbiotic mystery ofFrontiers in microbiology · 2025Review
- Article
- Multifaceted mitochondria in innate immunity.npj metabolic health and disease · 2024Review
- Protein sociology of ProA, Mip and other secreted virulence factors at theFrontiers in cellular and infection microbiology · 2023Review
- Risk assessment and quantitative measurement along with monitoring ofNew microbes and new infections · 2022Article
- The Functional Differences between the GroEL Chaperonin ofBiomolecules · 2021Article
- Pathogenicity and Virulence ofVirulence · 2021Review
- Genomic Characterization of Antimicrobial Resistance, Virulence, and Phylogeny of the GenusAntibiotics (Basel, Switzerland) · 2020Article
- Subcellular Location ofMicroorganisms · 2020Article
- Review
- Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii.Scientific reports · 2016Article
- IFNs Modify the Proteome of Legionella-Containing Vacuoles and Restrict Infection Via IRG1-Derived Itaconic Acid.PLoS pathogens · 2016Article
- Identification of vacuoles containing extraintestinal differentiated forms of Legionella pneumophila in colonized Caenorhabditis elegans soil nematodes.MicrobiologyOpen · 2015Article
- Article
- The many forms of a pleomorphic bacterial pathogen-the developmental network of Legionella pneumophila.Frontiers in microbiology · 2014Review
- Nutrient salvaging and metabolism by the intracellular pathogen Legionella pneumophila.Frontiers in cellular and infection microbiology · 2014Review
- Acquired Fanconi syndrome in patients with Legionella pneumonia.BMC nephrology · 2013Article
- Cell biology of infection by Legionella pneumophila.Microbes and infection · 2013Review
- Legionella pneumophila infection of Drosophila S2 cells induces only minor changes in mitochondrial dynamics.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A portion of the total cellular pool of the Legionella pneumophila chaperonin, HtpB, is found on the bacterial cell surface, where it can mediate invasion of nonphagocytic cells. HtpB continues to be abundantly produced and released by internalized L. pneumophila and may thus have postinvasion functions. We used here two functional models (protein-coated beads and expression of recombinant proteins in CHO cells) to investigate the competence of HtpB in mimicking early intracellular trafficking events of L. pneumophila, including the recruitment of mitochondria, cytoskeletal alterations, the inhibition of phagosome-lysosome fusion, and association with the endoplasmic reticulum. Microscopy and flow cytometry studies indicated that HtpB-coated beads recruited mitochondria in CHO cells and U937-derived macrophages and induced transient changes in the organization of actin microfilaments in CHO cells. Ectopic expression of HtpB in the cytoplasm of transfected CHO cells also led to modifications in actin microfilaments similar to those produced by HtpB-coated beads but did not change the distribution of mitochondria. Association of phagosomes containing HtpB-coated beads with the endoplasmic reticulum was not consistently detected by either fluorescence or electron microscopy studies, and only a modest delay in the fusion of TrOv-labeled lysosomes with phagosomes containing HtpB-coated beads was observed. HtpB is the first Legionella protein and the first chaperonin shown to, by means of our functional models, induce mitochondrial recruitment and microfilament rearrangements, two postinternalization events that typify the early trafficking of virulent L. pneumophila.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.