ArticleThe Journal of physiology2009
Expression of anoctamin 1/TMEM16A by interstitial cells of Cajal is fundamental for slow wave activity in gastrointestinal muscles.
Article in The Journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 249 papers.
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Who cites it
249 citing papers in PubMed, 419 citations in OpenAlex.
- Pacemaking in the lymphatic system.The Journal of physiology · 2026Review
- Cells and ionic conductances contributing to spontaneous activity in bladder and urethral smooth muscle.The Journal of physiology · 2026Review
- Viniferin and its derivatives: a comprehensive review of structural variations and promising pharmacological applications in disease prevention and therapeutic development.Naunyn-Schmiedeberg's archives of pharmacology · 2026Review
- The Function ofbioRxiv : the preprint server for biology · 2026Article
- Blockade of calcium-activated chloride channel ANO1 ameliorates ionizing radiation-induced intestinal injury.Journal of advanced research · 2026Article
- Role of ANO1 CaPflugers Archiv : European journal of physiology · 2025Article
- ER morphological analysis associated with interstitial cells of Cajal and smooth muscle cells in the murine stomach.Cell and tissue research · 2025Article
- Neuronal regulation of myenteric interstitial cells of Cajal (ICC-MY) in the proximal colon.Cell calcium · 2025Article
- Intramural Blood Vessels as a Primary Site of Vascular LUTS.Lower urinary tract symptoms · 2025Review
- LIPUS Enhances Gallbladder Motility via ANO1 in Acute Cholecystitis Guinea Pigs.Bioengineering (Basel, Switzerland) · 2025Article
- TMEM16A in gastrointestinal and vascular smooth muscle contractility.The Journal of physiology · 2025Review
- Article
- Differential responses to prostaglandins in the circular and longitudinal muscle layers of the murine ileum.American journal of physiology. Gastrointestinal and liver physiology · 2025Article
- ANO1 channels are expressed in mouse urethral smooth muscle but do not contribute to agonist or neurally evoked contractions.Scientific reports · 2025Article
- A specialized population of intramuscular interstitial cells of Cajal regulates motility within the mouse oesophagogastric junction.The Journal of physiology · 2025Article
- Article
- Ca²⁺ signaling in myenteric interstitial cells of Cajal (ICC-MY) and their role as conditional pacemakers in the colon.Cell calcium · 2025Article
- ANO1: central role and clinical significance in non-neoplastic and neoplastic diseases.Frontiers in immunology · 2025Review
- Immunohistochemical characterization of interstitial cells and their spatial relationship to motor neurons within the mouse esophagus.Cell and tissue research · 2025Article
- Evaluating the molecular function of zebrafish Anoctamin 1a.microPublication biology · 2025Article
189 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Interstitial cells of Cajal (ICC) generate pacemaker activity (slow waves) in gastrointestinal (GI) smooth muscles, but the mechanism(s) of pacemaker activity are controversial. Several conductances, such as Ca(2+)-activated Cl() channels (CaCC) and non-selective cation channels (NSCC) have been suggested to be involved in slow wave depolarization. We investigated the expression and function of a new class of CaCC, anoctamin 1 (ANO1), encoded by Tmem16a, which was discovered to be highly expressed in ICC in a microarray screen. GI muscles express splice variants of the Tmem16a transcript in addition to other paralogues of the Tmem16a family. ANO1 protein is expressed abundantly and specifically in ICC in all regions of the murine, non-human primate (Macaca fascicularis) and human GI tracts. CaCC blocking drugs, niflumic acid and 4,4-diisothiocyano-2,2-stillbene-disulfonic acid (DIDS) reduced the frequency and blocked slow waves in murine, primate, human small intestine and stomach in a concentration-dependent manner. Unitary potentials, small stochastic membrane depolarizations thought to underlie slow waves, were insensitive to CaCC blockers. Slow waves failed to develop by birth in mice homozygous for a null allele of Tmem16a (Tmem16a(tm1Bdh)(/tm1Bdh)) and did not develop subsequent to birth in organ culture, as in wildtype and heterozygous muscles. Loss of function of ANO1 did not inhibit the development of ICC networks that appeared structurally normal as indicated by Kit antibodies. These data demonstrate the fundamental role of ANO1 in the generation of slow waves in GI ICC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.