ArticleBiophysical journal2009
Single-molecule microscopy reveals membrane microdomain organization of cells in a living vertebrate.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- Analysis of the H-Ras mobility pattern in vivo shows cellular heterogeneity inside epidermal tissue.Disease models & mechanisms · 2022Article
- Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy.Biomedical optics express · 2021Article
- Single-Molecule Tracking of Chromatin-Associated Proteins in theThe journal of physical chemistry. B · 2021Article
- Single-molecule imaging correlates decreasing nuclear volume with increasing TF-chromatin associations during zebrafish development.Nature communications · 2018Article
- Fluorescence techniques in developmental biology.Journal of biosciences · 2018Review
- Cross-Scale Integrin Regulation Organizes ECM and Tissue Topology.Developmental cell · 2015Article
- Application of single molecule fluorescence microscopy to characterize the penetration of a large amphiphilic molecule in the stratum corneum of human skin.International journal of molecular sciences · 2015Article
- Nanoscale imaging of caveolin-1 membrane domains in vivo.PloS one · 2015Article
- Depth-of-Focus Correction in Single-Molecule Data Allows Analysis of 3D Diffusion of the Glucocorticoid Receptor in the Nucleus.PloS one · 2015Article
- In vivo single-molecule imaging identifies altered dynamics of calcium channels in dystrophin-mutant C. elegans.Nature communications · 2014Article
- Endocytosis and signaling during development.Cold Spring Harbor perspectives in biology · 2014Article
- Advances in genomics of bony fish.Briefings in functional genomics · 2014Review
- Single-molecule detection and tracking in plants.Protoplasma · 2014Review
- Quantitation of glucocorticoid receptor DNA-binding dynamics by single-molecule microscopy and FRAP.PloS one · 2014Article
- Quantifying the dynamic interactions between a clathrin-coated pit and cargo molecules.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Inferring diffusion in single live cells at the single-molecule level.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Single molecule fluorescence detection and tracking in mammalian cells: the state-of-the-art and future perspectives.International journal of molecular sciences · 2012Review
- Membrane cholesterol strongly influences confined diffusion of prestin.Biophysical journal · 2012Article
- Automated multidimensional single molecule fluorescence microscopy feature detection and tracking.European biophysics journal : EBJ · 2011Article
- Dose- and time-dependent expression patterns of zebrafish orthologs of selected E2F target genes in response to serum starvation/replenishment.Molecular biology reports · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been possible for several years to study the dynamics of fluorescently labeled proteins by single-molecule microscopy, but until now this technology has been applied only to individual cells in culture. In this study, it was extended to stem cells and living vertebrate organisms. As a molecule of interest we used yellow fluorescent protein fused to the human H-Ras membrane anchor, which has been shown to serve as a model for proteins anchored in the plasma membrane. We used a wide-field fluorescence microscopy setup to visualize individual molecules in a zebrafish cell line (ZF4) and in primary embryonic stem cells. A total-internal-reflection microscopy setup was used for imaging in living organisms, in particular in epidermal cells in the skin of 2-day-old zebrafish embryos. Our results demonstrate the occurrence of membrane microdomains in which the diffusion of membrane proteins in a living organism is confined. This membrane organization differed significantly from that observed in cultured cells, illustrating the relevance of performing single-molecule microscopy in living organisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.