ArticleBiophysical journal2009
DNA deformations near charged surfaces: electron and atomic force microscopy views.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- PS Poly: A chain tracing algorithm to determine persistence length and categorize complex polymers by shape.PloS one · 2026Article
- Polymer Length Governs DNA Adsorption Dynamics on Mineral Surfaces.Environmental science & technology · 2025Article
- Approaches for Determining DNA Persistence Length Using Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Mechanical Constraint Effect on DNA Persistence Length.Molecules (Basel, Switzerland) · 2022Article
- Atomic force microscopy-A tool for structural and translational DNA research.APL bioengineering · 2021Review
- AutoSmarTrace: Automated chain tracing and flexibility analysis of biological filaments.Biophysical journal · 2021Article
- Mechanical Flexibility of DNA: A Quintessential Tool for DNA Nanotechnology.Sensors (Basel, Switzerland) · 2020Review
- Automated AFM analysis of DNA bending reveals initial lesion sensing strategies of DNA glycosylases.Scientific reports · 2020Article
- Nanomechanics of Diaminopurine-Substituted DNA.Biophysical journal · 2019Article
- Identification of Extrachromosomal Linear microDNAs Interacted with microRNAs in the Cell Nuclei.Cells · 2019Article
- Environmentally Controlled Curvature of Single Collagen Proteins.Biophysical journal · 2018Article
- C-Terminal Truncated α-Synuclein Fibrils Contain Strongly Twisted β-Sheets.Journal of the American Chemical Society · 2017Article
- The Impact of N-terminal Acetylation of α-Synuclein on Phospholipid Membrane Binding and Fibril Structure.The Journal of biological chemistry · 2016Article
- Atomic force microscopy study of DNA flexibility on short length scales: smooth bending versus kinking.Nucleic acids research · 2014Article
- Symmetric curvature descriptors for label-free analysis of DNA.Scientific reports · 2014Article
- Mechanical properties of base-modified DNA are not strictly determined by base stacking or electrostatic interactions.Biophysical journal · 2014Article
- Label-free, atomic force microscopy-based mapping of DNA intrinsic curvature for the nanoscale comparative analysis of bent duplexes.Nucleic acids research · 2012Article
- Direct measurement of DNA bending by type IIA topoisomerases: implications for non-equilibrium topology simplification.Nucleic acids research · 2011Article
- Cross-seeding of alpha-synuclein aggregation by amyloid fibrils of food proteins.The Journal of biological chemistryArticle
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
DNA is a very important cell structural element, which determines the level of expression of genes by virtue of its interaction with regulatory proteins. We use electron (EM) and atomic force microscopy (AFM) to characterize the flexibility of double-stranded DNA ( approximately 150-950 nm long) close to a charged surface. Automated procedures for the extraction of DNA contours ( approximately 10-120 nm for EM data and approximately 10-300 nm for AFM data) combined with new statistical chain descriptors indicate a uniquely two-dimensional equilibration of the molecules on the substrate surface regardless of the procedure of molecule mounting. However, in contrast to AFM, the EM mounting leads to a noticeable decrease in DNA persistence length together with decreased kurtosis. Analysis of local bending on short length scales (down to 6 nm in the EM study) shows that DNA flexibility behaves as predicted by the wormlike chain model. We therefore argue that adhesion of DNA to a charged surface may lead to additional static bending (kinking) of approximately 5 degrees per dinucleotide step without impairing the dynamic behavior of the DNA backbone. Implications of this finding are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.