ArticleBiophysical journal2009
Real-time monitoring of transferrin-induced endocytic vesicle formation by mid-infrared surface plasmon resonance.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- SPR Sensing: From Biomolecular Interactions to Cell-Based Analysis.Biosensors · 2026Review
- Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance.Sensors (Basel, Switzerland) · 2021Review
- Quantitative label-free imaging of iron-bound transferrin in breast cancer cells and tumors.Redox biology · 2020Article
- The Convergence of Cell-Based Surface Plasmon Resonance and Biomaterials: The Future of Quantifying Bio-molecular Interactions-A Review.Annals of biomedical engineering · 2020Review
- Triangular correlation (TrC) between cancer aggressiveness, cell uptake capability, and cell deformability.Science advances · 2020Article
- Real-Time Label-Free Targeting Assessment and in Vitro Characterization of Curcumin-Loaded Poly-lactic-ACS omega · 2019Article
- A Localized Surface Plasmon Resonance Sensor Using Double-Metal-Complex Nanostructures and a Review of Recent Approaches.Sensors (Basel, Switzerland) · 2017Review
- Poly-arginine and arginine-rich peptides are neuroprotective in stroke models.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2015Article
- Transferrin receptor-mediated endocytosis: a useful target for cancer therapy.The Journal of membrane biology · 2014Review
- Surface plasmon resonance for cell-based clinical diagnosis.Sensors (Basel, Switzerland) · 2014Review
- Bioanalysis of eukaryotic organelles.Chemical reviews · 2013Review
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- Article
- The intracellular trafficking pathway of transferrin.Biochimica et biophysica acta · 2012Review
- Real-time monitoring of epithelial cell-cell and cell-substrate interactions by infrared surface plasmon spectroscopy.Biophysical journal · 2010Article
- SRC-mediated phosphorylation of dynamin and cortactin regulates the "constitutive" endocytosis of transferrin.Molecular and cellular biology · 2010Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on the application of surface plasmon resonance (SPR), based on Fourier transform infrared spectroscopy in the mid-infrared wavelength range, for real-time and label-free sensing of transferrin-induced endocytic processes in human melanoma cells. The evanescent field of the mid-infrared surface plasmon penetrates deep into the cell, allowing highly sensitive SPR measurements of dynamic processes occurring at significant cellular depths. We monitored in real-time, infrared reflectivity spectra in the SPR regime from living cells exposed to human transferrin (Tfn). We show that although fluorescence microscopy measures primarily Tfn accumulation in recycling endosomes located deep in the cell's cytoplasm, the SPR technique measures mainly Tfn-mediated formation of early endocytic organelles located in close proximity to the plasma membrane. Our SPR and fluorescence data are very well described by a kinetic model of Tfn endocytosis, suggested previously in similar cell systems. Hence, our SPR data provide further support to the rather controversial ability of Tfn to stimulate its own endocytosis. Our analysis also yields what we believe is novel information on the role of membrane cholesterol in modulating the kinetics of endocytic vesicle biogenesis and consumption.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.