Evidence map›Paper›PMID 19686638›Full record

ArticleBiophysical journal2009

DySCo: quantitating associations of membrane proteins using two-color single-molecule tracking.

Paul D Dunne, Ricardo A Fernandes, James McColl, Ji Won Yoon, John R James, Simon J Davis, David Klenerman

Abstract readCommentLetter
In one paragraph

Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed.

  1. Pre-organized landscape of T cell surface.Frontiers in immunology · 2023
    Review
  2. Article
  3. Trapping or slowing the diffusion of T cell receptors at close contacts initiates T cell signaling.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Membrane Ultrastructure and T Cell Activation.Frontiers in immunology · 2018
    Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Stoichiometry and assembly of mTOR complexes revealed by single-molecule pulldown.Proceedings of the National Academy of Sciences of the United States of America · 2014
    Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Paul D Dunne
Ricardo A Fernandes
James McColl
Ji Won Yoon
John R James
Simon J Davis
David Klenerman

Funding

Biotechnology and Biological Sciences Research Council BB/D000467/1Medical Research CouncilWellcome Trust 081894
6 · The paper itself

Abstract

We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small.

Indexed as

Equipment DesignEquipment Failure AnalysisFluorescent DyesMicroscopy, Fluorescence, MultiphotonMolecular Probe TechniquesProtein Interaction MappingReproducibility of ResultsSensitivity and SpecificityFluorescent Dyes

Identifiers

PMID19686638
PMCPMC2726305

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.