ArticlePharmaceutical research2009
Mutagenesis and cysteine scanning of transmembrane domain 10 of the human dipeptide transporter.
Article in Pharmaceutical research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- G protein-coupled receptors: A target for microbial metabolites and a mechanistic link to microbiome-immune-brain interactions.Brain, behavior, & immunity - health · 2023Review
- Extracellular domain of PepT1 interacts with TM1 to facilitate substrate transport.Structure (London, England : 1993) · 2022Article
- Proton coupling and the multiscale kinetic mechanism of a peptide transporter.Biophysical journal · 2022Article
- Chemical Modulation of the Human Oligopeptide Transporter 1, hPepT1.Molecular pharmaceutics · 2017Article
- Metastatic triple-negative breast cancer patient withCold Spring Harbor molecular case studies · 2017Article
- Major osteoporotic fragility fractures: Risk factor updates and societal impact.World journal of orthopedics · 2016 · on this mapReview
- Molecular insights into proton coupled peptide transport in the PTR family of oligopeptide transporters.Biochimica et biophysica acta · 2015Review
- Structural insights into substrate recognition in proton-dependent oligopeptide transporters.EMBO reports · 2013Article
- Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Alternating access mechanism in the POT family of oligopeptide transporters.The EMBO journal · 2012Article
- Probing the putative active site of YjdL: an unusual proton-coupled oligopeptide transporter from E. coli.PloS one · 2012Article
- Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2.The EMBO journal · 2011Article
- Functional role of the intracellular loop linking transmembrane domains 6 and 7 of the human dipeptide transporter hPEPT1.The Journal of membrane biology · 2010Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
purposeThe human dipeptide transporter (hPEPT1) facilitates transport of dipeptides and drugs from the intestine into the circulation. The role of transmembrane domain 10 (TMD10) of hPEPT1 in substrate translocation was investigated using cysteine-scanning mutagenesis with 2-Trimethylammonioethyl methanethiosulfonate (MTSET).
methodsEach amino acid in TMD10 was mutated individually to cysteine, and transport of [(3)H]Gly-Sar was evaluated with and without MTSET following transfection of each mutant in HEK293 cells. Similar localization and expression levels of wild type (WT) hPEPT1 and all mutants were confirmed by immunostaining and biotinylation followed by western blot analysis.
resultsE595C- and G594C-hPEPT1 showed negligible Gly-Sar uptake. E595D-hPEPT1 showed similar uptake to WT-hPEPT1, but E595K- and E595R-hPEPT1 did not transport Gly-Sar. Double mutations E595K/R282E and E595R/R282E did not restore uptake. G594A-hPEPT1 showed similar uptake to WT-hPEPT1, but G594V-hPEPT1 eliminated uptake. Y588C-hPEPT1 showed uptake of 20% that of WT-hPEPT1. MTSET modification supported a model of TMD10 with an amphipathic helix from I585 to V600 and increased solvent accessibility from T601 to F605.
conclusionsOur results suggest that G594 and E595 in TMD10 of hPEPT1 have key roles in substrate transport and that Y588 may have an important secondary mechanistic role.
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