ReviewCellular and molecular life sciences : CMLS2009
Expressed protein ligation: a resourceful tool to study protein structure and function.
Review in Cellular and molecular life sciences : CMLS, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 69 citations in OpenAlex.
- Ligation of multiple protein domains using orthogonal inteins with non-native splice junctions.Protein science : a publication of the Protein Society · 2024Article
- Enhancing the performance of a mutant pyrrolysyl-tRNA synthetase to create a highly versatile eukaryotic cell-free protein synthesis tool.Scientific reports · 2023Article
- Development and Recent Advances in Lysine and N-Terminal Bioconjugation for Peptides and Proteins.Molecules (Basel, Switzerland) · 2023Review
- Fungicidal Activity of Recombinant Javanicin againstMolecules (Basel, Switzerland) · 2021Article
- Conjugation of a Blood Brain Barrier Peptide Shuttle to an Fc Domain for Brain Delivery of Therapeutic Biomolecules.ACS medicinal chemistry letters · 2021Article
- Bioorthogonal Reactions of Triarylphosphines and Related Analogues.Chemical reviews · 2021Review
- Deuterium-Enhanced Raman Spectroscopy for Histidine pKBiophysical journal · 2020Article
- Traceless native chemical ligation of lipid-modified peptide surfactants by mixed micelle formation.Nature communications · 2020Article
- Methods to Study the Structure and Catalytic Activity of cis-Splicing Inteins.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Recombinant Expression of Cyclotides Using Expressed Protein Ligation.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Strategies for Generating Modified Nucleosomes: Applications within Structural Biology Studies.ACS chemical biology · 2019Review
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
- An explorative study towards the chemical synthesis of the immunoglobulin G1 Fc CH3 domain.Journal of peptide science : an official publication of the European Peptide Society · 2018Article
- In-cell production of a genetically-encoded library based on the θ-defensin RTD-1 using a bacterial expression system.Bioorganic & medicinal chemistry · 2018Article
- Chemical Synthesis of GPI Glycan-Peptide Conjugates by Traceless Staudinger Ligation.Organic letters · 2017Article
- Exploring chemoselective S-to-N acyl transfer reactions in synthesis and chemical biology.Nature communications · 2017Review
- Advances in nano-based inner ear delivery systems for the treatment of sensorineural hearing loss.Advanced drug delivery reviews · 2017Review
- Article
- Biophysical Characterization of Fluorotyrosine Probes Site-Specifically Incorporated into Enzymes: E. coli Ribonucleotide Reductase As an Example.Journal of the American Chemical Society · 2016Article
- Intein applications: from protein purification and labeling to metabolic control methods.The Journal of biological chemistry · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This review outlines the use of expressed protein ligation (EPL) to study protein structure, function and stability. EPL is a chemoselective ligation method that allows the selective ligation of unprotected polypeptides from synthetic and recombinant origin for the production of semi-synthetic protein samples of well-defined and homogeneous chemical composition. This method has been extensively used for the site-specific introduction of biophysical probes, unnatural amino acids, and increasingly complex post-translational modifications. Since it was introduced 10 years ago, EPL applications have grown increasingly more sophisticated in order to address even more complex biological questions. In this review, we highlight how this powerful technology combined with standard biochemical analysis techniques has been used to improve our ability to understand protein structure and function.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.