ArticleApplied and environmental microbiology2009
Cell-free Escherichia coli-based system to screen for quorum-sensing molecules interacting with quorum receptor proteins of Streptomyces coelicolor.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Emerging advances in biosensor technologies for quorum sensing signal molecules.Analytical and bioanalytical chemistry · 2025Review
- Transcription Factor-Based Biosensors for Detecting Pathogens.Biosensors · 2022Review
- Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors.Biosensors · 2022Review
- A Glossary for Chemical Approaches towards Unlocking the Trove of Metabolic Treasures inMolecules (Basel, Switzerland) · 2021Review
- Portable environment-signal detection biosensors with cell-free synthetic biosystems.RSC advances · 2020Article
- Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences.Frontiers in microbiology · 2020Review
- Regulation of Antibiotic Production by Signaling Molecules inFrontiers in microbiology · 2019Review
- Review
- Quorum Sensing: An Under-Explored Phenomenon in the Phylum Actinobacteria.Frontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quorum sensing (QS) is mediated by small molecules and involved in diverse cellular functions, such as virulence, biofilm formation, secondary metabolism, and cell differentiation. In this study, we developed a rapid and effective screening tool based on a cell-free Escherichia coli-based expression system to identify QS molecules of Streptomyces. The binding of QS molecules to gamma-butyrolactone receptor ScbR was monitored by changes in the expression levels of the green fluorescent protein reporter in E. coli cell extract. Using this assay system, we could successfully confirm SCB1, a gamma-butyrolactone molecule in Streptomyces coelicolor, binding to its known receptor, ScbR. In addition, we have shown that N-hexanoyl-DL-homoserine lactone, one of the QS molecules in many gram-negative bacteria, can regulate ScbR and trigger precocious antibiotic production in S. coelicolor. Our new method can be applied to other strains for which a screening tool for QS molecules has not yet been developed.
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Registered trials
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