ArticleApplied and environmental microbiology2009
Acetolactate synthase from Bacillus subtilis serves as a 2-ketoisovalerate decarboxylase for isobutanol biosynthesis in Escherichia coli.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 107 citations in OpenAlex.
- Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability.Biotechnology for biofuels and bioproducts · 2026Review
- Microbial synthesis of branched-chain β,γ-diols from amino acid metabolism.Nature communications · 2025Article
- Engineering the carbon and redox metabolism of Paenibacillus polymyxa for efficient isobutanol production.Microbial biotechnology · 2024Article
- Biosynthesis of Diverse Ephedra-Type Alkaloids via a Newly Identified Enzymatic Cascade.Biodesign research · 2024Article
- Biochemical and Structural Insights into a Thiamine Diphosphate-Dependent α-Ketoglutarate Decarboxylase from CyanobacteriumInternational journal of molecular sciences · 2023Article
- Article
- Evaluation of Metabolic Engineering Strategies on 2-Ketoisovalerate Production by Escherichia coli.Applied and environmental microbiology · 2022Article
- The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae.Biotechnology for biofuels and bioproducts · 2022Article
- Efficient Large-Scale and Scarless Genome Engineering Enables the Construction and Screening ofInternational journal of molecular sciences · 2022Article
- Expressing 2-keto acid pathway enzymes significantly increases photosynthetic isobutanol production.Microbial cell factories · 2022Article
- Microbial Lipopeptide-Producing Strains and Their Metabolic Roles under Anaerobic Conditions.Microorganisms · 2021Review
- Metabolic engineering of Escherichia coli for the production of isobutanol: a review.World journal of microbiology & biotechnology · 2021Review
- Algal glycobiotechnology: omics approaches for strain improvement.Microbial cell factories · 2021Review
- Pyruvate Production by Escherichia coli by Use of Pyruvate Dehydrogenase Variants.Applied and environmental microbiology · 2021Article
- Metabolic engineering of Escherichia coli W for isobutanol production on chemically defined medium and cheese whey as alternative raw material.Journal of industrial microbiology & biotechnology · 2020Article
- A Genome-Scale Metabolic Model of 2,3-Butanediol Production by Thermophilic BacteriaMicroorganisms · 2020Article
- Structure-Based Design of Acetolactate Synthase FromFrontiers in microbiology · 2020Article
- Protein-based biorefining driven by nitrogen-responsive transcriptional machinery.Biotechnology for biofuels · 2020Article
- Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method.Microbial cell factories · 2019Article
- A synthetic pathway for the production of 2-hydroxyisovaleric acid in Escherichia coli.Journal of industrial microbiology & biotechnology · 2018Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A pathway toward isobutanol production previously constructed in Escherichia coli involves 2-ketoacid decarboxylase (Kdc) from Lactococcus lactis that decarboxylates 2-ketoisovalerate (KIV) to isobutyraldehyde. Here, we showed that a strain lacking Kdc is still capable of producing isobutanol. We found that acetolactate synthase from Bacillus subtilis (AlsS), which originally catalyzes the condensation of two molecules of pyruvate to form 2-acetolactate, is able to catalyze the decarboxylation of KIV like Kdc both in vivo and in vitro. Mutational studies revealed that the replacement of Q487 with amino acids with small side chains (Ala, Ser, and Gly) diminished only the decarboxylase activity but maintained the synthase activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.