ArticleGene2009
Functional characterization of PAS and HES family bHLH transcription factors during the metamorphosis of the red flour beetle, Tribolium castaneum.
Article in Gene, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- Molecular mechanisms underlying metamorphosis in the most-ancestral winged insect.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Importance of Taiman in Larval-Pupal Transition inFrontiers in physiology · 2019Article
- Identification and Phylogenetic Analysis of Basic Helix-Loop-Helix Genes in the Diamondback Moth.Journal of insect science (Online) · 2018Article
- Article
- A role for Taiman in insect metamorphosis.PLoS genetics · 2014Article
- Juvenile hormone regulates Aedes aegypti Krüppel homolog 1 through a conserved E box motif.Insect biochemistry and molecular biology · 2014Article
- Role of Methoprene-tolerant (Met) in adult morphogenesis and in adult ecdysis of Blattella germanica.PloS one · 2014Article
- Article
- A genome-wide identification of basic helix-loop-helix motifs in Pediculus humanus corporis (Phthiraptera: Pediculidae).Journal of insect science (Online) · 2014Article
- Establishment of a versatile cell line for juvenile hormone signaling analysis in Tribolium castaneum.Scientific reports · 2013Article
- Review
- HLH-29 regulates ovulation in C. elegans by targeting genes in the inositol triphosphate signaling pathway.Biology open · 2012Article
- Two adjacent cis-regulatory elements are required for ecdysone response of ecdysone receptor (EcR) B1 transcription.PloS one · 2012Article
- Large-scale RNAi screen of G protein-coupled receptors involved in larval growth, molting and metamorphosis in the red flour beetle.BMC genomics · 2011Article
- Steroid receptor co-activator is required for juvenile hormone signal transduction through a bHLH-PAS transcription factor, methoprene tolerant.The Journal of biological chemistry · 2011Article
- Molecular cloning and characterization of twist gene in Bombyx mori.Molecular and cellular biochemistry · 2011Article
- The members of bHLH transcription factor superfamily are required for female reproduction in the red flour beetle, Tribolium castaneum.Journal of insect physiology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The basic helix-loop-helix transcription factors are present in animals, plants and fungi and play important roles in the control of cellular proliferation, tissue differentiation, development and detoxification. Although insect genomes contain more than 50 helix-loop-helix transcription factors, the functions of only a few are known. RNAi has become a widely used tool to knock-down the expression to analyze the function of genes. As RNAi works well in Tribolium castaneum, we utilized this insect and RNAi to determine functions of 19 bHLH transcription factors belonging to PAS and HES families during the larval stages of the red flour beetle, T. castaneum. We searched the genome sequence of T. castaneum and identified 53 bHLH genes. Phylogenetic analyses classified these 53 genes into ten families; PAS, HES, Myc/USF, Hand, Mesp, Shout, p48, NeuroD/Neurogenin, Atonal and AS-C. In RNAi studies, knocking-down the expression of seven members of the PAS and HES families affected the growth and development of T. castaneum. An inability to grow to reach critical weight to undergo metamorphosis, failure to complete larval-pupal or pupal-adult ecdysis and abnormal wing development are among the most common phenotypes observed in RNAi insects. Among the bHLH transcription factors studied, the steroid receptor coactivator (SRC) showed the most severe phenotypes. Knock-down in the expression of the gene coding for SRC caused growth arrest by affecting the regulation of lipid metabolism. These studies demonstrate the power of RNAi for functional characterization of members of the multigene families in this model insect.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.