ArticleVirus research2009
Characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27.
Article in Virus research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The functions of herpesvirus shuttling proteins in the virus lifecycle.Frontiers in microbiology · 2025Review
- Progress in the study of parvovirus entry pathway.Virology journal · 2023Review
- Nucleocytoplasmic Shuttling of Porcine Parvovirus NS1 Protein Mediated by the CRM1 Nuclear Export Pathway and the Importin α/β Nuclear Import Pathway.Journal of virology · 2022Article
- Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein.Virology journal · 2017Article
- Duck enteritis virus UL54 is an IE protein primarily located in the nucleus.Virology journal · 2015Article
- Article
- Identification of a novel nuclear localization signal sequence in Chlamydia trachomatis-secreted hypothetical protein CT311.PloS one · 2013Article
- Article
- ICP27 protein encoded by bovine herpesvirus type 1 (bICP27) interferes with promoter activity of the bovine genes encoding beta interferon 1 (IFN-β1) and IFN-β3.Virus research · 2012Article
- A PY-nuclear localization signal is required for nuclear accumulation of HCMV UL79 protein.Medical microbiology and immunology · 2012Article
- Molecular determinants responsible for the subcellular localization of HSV-1 UL4 protein.Virologica Sinica · 2011Article
- Identification of nuclear and nucleolar localization signals of pseudorabies virus (PRV) early protein UL54 reveals that its nuclear targeting is required for efficient production of PRV.Journal of virology · 2011Article
- Screening and identification of host factors interacting with UL14 of herpes simplex virus 1.Medical microbiology and immunology · 2011Article
- Identification of a linear epitope on the capsid protein of classical swine fever virus.Virus research · 2011Article
- Molecular anatomy of subcellular localization of HSV-1 tegument protein US11 in living cells.Virus research · 2010Article
- The nucleolus and viral infection.Virologica Sinica · 2010Review
- The nucleocytoplasmic transport of viral proteins.Virologica Sinica · 2010Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine herpesvirus-1 infected cell protein 27 (BICP27) was detected predominantly in the nucleolus. The open reading frame of BICP27 was fused with the enhanced yellow fluorescent protein (EYFP) gene to investigate its subcellular localization in live cells and BICP27 was able to direct monomeric, dimeric or trimeric EYFP exclusively to the nucleolus. By constructing a series of deletion mutants, the putative nuclear localization signal (NLS) and nucleolar localization signal (NoLS) were mapped to (81)RRAR(84) and (86)RPRRPRRRPRRR(97) respectively. Specific deletion of the putative NLS, NoLS or both abrogated nuclear localization, nucleolar localization or both respectively. Furthermore, NLS was able to direct trimeric EYFP predominantly to the nucleus but excluded from the nucleolus, whereas NoLS targeted trimeric EYFP primarily to the nucleus, and enriched in the nucleolus with faint staining in the cytoplasm. NLS+NoLS directed trimeric EYFP predominantly to the nucleolus with faint staining in the nucleus. Moreover, deletion of NLS+NoLS abolished the transactivating activity of BICP27 on gC promoter, whereas deletion of either NLS or NoLS did not. The study demonstrated that BICP27 is a nucleolar protein, adding BICP27 to the growing list of transactivators which localize to the nucleolus.
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