ArticleMolecular microbiology2009
A mechanism of transposon-mediated directed mutation.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- Investigating the Trans Effects of IS<italic>1</italic> Transposases on Intragenomic DNA Movements in <italic>Escherichia coli</italic>.Microbial physiology · 2026Article
- Examining the Roles of Genomic Context and Endogenous Regulatory Elements on ISInternational journal of molecular sciences · 2025Article
- Transcriptional mechanism by which IS5 activates the fucAO operon in Escherichia coli.Nucleic acids research · 2025Article
- Investigating How Genomic Contexts Impact IS5 Transposition Within theMicroorganisms · 2024Article
- Comprehensive Characterization ofInternational journal of molecular sciences · 2024Article
- The effect of DNA-binding proteins on insertion sequence element transposition upstream of theFrontiers in microbiology · 2024Article
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- Article
- Effects of Global and Specific DNA-Binding Proteins on Transcriptional Regulation of theInternational journal of molecular sciences · 2022Article
- Insertion Sequence (IS) Element-Mediated Activating Mutations of the Cryptic Aromatic β-Glucoside Utilization (International journal of molecular sciences · 2022Article
- Histone-like nucleoid structuring (H-NS) protein silences the beta-glucoside (Computational and structural biotechnology journal · 2022Article
- Environment-directed activation of the Escherichia coliflhDC operon by transposons.Microbiology (Reading, England) · 2017Article
- Transposon-mediated directed mutation in bacteria and eukaryotes.Frontiers in bioscience (Landmark edition) · 2017Review
- Hopping into a hot seat: Role of DNA structural features on IS5-mediated gene activation and inactivation under stress.PloS one · 2017Article
- Disentangling genetic and epigenetic determinants of ultrafast adaptation.Molecular systems biology · 2016Article
- Zinc-Induced Transposition of Insertion Sequence Elements Contributes to Increased Adaptability of Cupriavidus metallidurans.Frontiers in microbiology · 2016Article
- The Bacterial Phosphotransferase System: New Frontiers 50 Years after Its Discovery.Journal of molecular microbiology and biotechnology · 2015Article
- Control of Transposon-Mediated Directed Mutation by the Escherichia coli Phosphoenolpyruvate:Sugar Phosphotransferase System.Journal of molecular microbiology and biotechnology · 2015Review
- Transposon-mediated directed mutation controlled by DNA binding proteins in Escherichia coli.Frontiers in microbiology · 2014Article
- Utilization of D-ribitol by Lactobacillus casei BL23 requires a mannose-type phosphotransferase system and three catabolic enzymes.Journal of bacteriology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Directed mutation is a proposed process that allows mutations to occur at higher frequencies when they are beneficial. Until now, the existence of such a process has been controversial. Here we describe a novel mechanism of directed mutation mediated by the transposon, IS5 in Escherichia coli. crp deletion mutants mutate specifically to glycerol utilization (Glp(+)) at rates that are enhanced by glycerol or the loss of the glycerol repressor (GlpR), depressed by glucose or glpR overexpression, and RecA-independent. Of the four tandem GlpR binding sites (O1-O4) upstream of the glpFK operon, O4 specifically controls glpFK expression while O1 primarily controls mutation rate in a process mediated by IS5 hopping to a specific site on the E. coli chromosome upstream of the glpFK promoter. IS5 insertion into other gene activation sites is unaffected by the presence of glycerol or the loss of GlpR. The results establish an example of transposon-mediated directed mutation, identify the protein responsible and define the mechanism involved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.