ArticleAnalytical chemistry2009
Antibody selection for immobilizing living bacteria.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 37 citations in OpenAlex.
- Enhancing Antigen Recognition of Living Cells by Site-Specific VHH Antibody Conjugation.Chembiochem : a European journal of chemical biology · 2025Article
- Long-Period Gratings and Microcavity In-Line Mach Zehnder Interferometers as Highly Sensitive Optical Fiber Platforms for Bacteria Sensing.Sensors (Basel, Switzerland) · 2020Review
- From molecules to multispecies ecosystems: the roles of structure in bacterial biofilms.Physical biology · 2019Review
- Detecting ligand interactions in real time on living bacterial cells.Applied microbiology and biotechnology · 2018Article
- Linking genetic variation in human Toll-like receptor 5 genes to the gut microbiome's potential to cause inflammation.Immunology letters · 2014Review
- Innate and adaptive immunity interact to quench microbiome flagellar motility in the gut.Cell host & microbe · 2013Article
- Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli.International journal of molecular sciences · 2013Article
- Role of overexpressed CFA/I fimbriae in bacterial swimming.Physical biology · 2012Article
- Capture efficiency of Escherichia coli in fimbriae-mediated immunoimmobilization.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Flagella overexpression attenuates Salmonella pathogenesis.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
We report a comparative study of the efficacy of immobilizing living bacteria by means of seven antibodies against bacterial surface antigens associated with Salmonella enterica Serovar Typhimurium. The targeted bacterial antigens were CFA/I fimbriae, flagella, lipopolysaccharides (LPS), and capsular F1 antigen. The best immobilization of S. Typhimurium was achieved with the antibody against CFA/I fimbriae. The immobilization of bacteria using antiflagellin showed significant enhancement if the flagella rotary motion was paralyzed. Of the four antibodies targeting LPS structures, only one, the antibody against the O-antigen polysaccharides, showed a relatively efficient bacterial immobilization. No bacterial immobilization was achieved using the antibody against F1 antigen, presumably because F1 protein can detach from the bacterial surface easily. The results suggest that an antibody for bacterial immunoimmobilization should target a surface antigen which extends out from the bacterial surface and is tightly attached to the bacterial cell wall. The microarrays of living S. Typhimurium cells immobilized in this manner remained viable and effective for at least 2 weeks in growth medium before a thick biofilm covered the whole surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.