ArticleEndocrine2009
Microarray analysis of altered gene expression in ERbeta-overexpressing HEK293 cells.
Article in Endocrine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Association ofCancers · 2021Article
- Low-Dose Bisphenol A in a Rat Model of Endometrial Cancer: A CLARITY-BPA Study.Environmental health perspectives · 2020Article
- Infant Formula Feeding Changes the Proliferative Status in Piglet Neonatal Mammary Glands Independently of Estrogen Signaling.The Journal of nutrition · 2020Article
- Keratin 86 is up-regulated in the uterus during implantation, induced by oestradiol.BMC developmental biology · 2020Article
- MicroRNA-382-5p aggravates breast cancer progression by regulating the RERG/Ras/ERK signaling axis.Oncotarget · 2017Article
- Editor's Highlight: Development of an In vitro Assay Measuring Uterine-Specific Estrogenic Responses for Use in Chemical Safety Assessment.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- The transcriptional coregulator MAML1 affects DNA methylation and gene expression patterns in human embryonic kidney cells.Molecular biology reports · 2016Article
- mRNA profiling reveals determinants of trastuzumab efficiency in HER2-positive breast cancer.PloS one · 2015Article
- Estrogen Receptor β Agonists Differentially Affect the Growth of Human Melanoma Cell Lines.PloS one · 2015Article
- ERβ- and prostaglandin E2-regulated pathways integrate cell proliferation via Ras-like and estrogen-regulated growth inhibitor in endometriosis.Molecular endocrinology (Baltimore, Md.) · 2014Article
- Research resource: global identification of estrogen receptor β target genes in triple negative breast cancer cells.Molecular endocrinology (Baltimore, Md.) · 2013Article
- ERβ-dependent effects on uterine endothelial cells are cell specific and mediated via Sp1.Human reproduction (Oxford, England) · 2013Article
- Identification of novel gene targets and functions of p21-activated kinase 1 during DNA damage by gene expression profiling.PloS one · 2013Article
- Mechanistic modeling of the effects of myoferlin on tumor cell invasion.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Estrogen signaling via estrogen receptor {beta}.The Journal of biological chemistry · 2010Review
- Adult rat bones maintain distinct regionalized expression of markers associated with their development.PloS one · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Estrogen receptors (ERs), ERalpha and ERbeta, mediate estrogen actions in a broad range of target tissues. With the introduction of microarray techniques, a significant understanding has been gained regarding the interplay between the ERalpha and ERbeta in breast cancer cell lines. To gain a more comprehensive understanding of ERbeta-dependent gene regulation independent of ERalpha, we performed microarray analysis on HEK293/mock and HEK293/ERbeta cells. A total of 332 genes was identified as ERbeta-upregulated genes and 210 identified as ERbeta-downregulated genes. ERbeta-induced and ERbeta-repressed genes were involved in cell-cell signaling, morphogenesis, and cell proliferation. The ERbeta repressive effect on genes related to proliferation was further studied by proliferation assays, where ERbeta expression resulted in a significant decrease in cell proliferation. To identify primary ERbeta target genes, we examined a number of ERbeta-regulated genes using chromatin immunoprecipitation assays for regions bound by ERbeta. Our results showed that ERbeta recruitment was significant to regions associated with 12 genes (IL1RAP, TMSB4X, COLEC12, ENPP2, KLRC1, RERG, RGS16, TNNT2, CYR61, FER1L3, FAM108A1, and CYP4X1), suggesting that these genes are likely to be ERbeta primary target genes. This study has provided novel information on the gene regulatory function of ERbeta independent of ERalpha and identified a number of ERbeta primary target genes. The results of Gene Ontology analysis and proliferation assays are consistent with an antiproliferative role of ERbeta independent of ERalpha.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.