ArticlePLoS genetics2009
The CUGBP2 splicing factor regulates an ensemble of branchpoints from perimeter binding sites with implications for autoregulation.
Article in PLoS genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Neuron-specific repression of alternative splicing by the conserved CELF protein UNC-75 in Caenorhabditis elegans.Genetics · 2025Article
- RNA Binding Protein CELF2 Regulates Signal-Induced Alternative Polyadenylation by Competing with Enhancers of the Polyadenylation Machinery.Cell reports · 2019Article
- Most human introns are recognized via multiple and tissue-specific branchpoints.Genes & development · 2018Article
- Ancient antagonism between CELF and RBFOX families tunes mRNA splicing outcomes.Genome research · 2017Article
- Article
- Position-dependent activity of CELF2 in the regulation of splicing and implications for signal-responsive regulation in T cells.RNA biology · 2016Article
- Diverse regulation of 3' splice site usage.Cellular and molecular life sciences : CMLS · 2015Review
- Antagonistic regulation of mRNA expression and splicing by CELF and MBNL proteins.Genome research · 2015Article
- Induced transcription and stability of CELF2 mRNA drives widespread alternative splicing during T-cell signaling.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A posttranscriptional mechanism that controls Ptbp1 abundance in the Xenopus epidermis.Molecular and cellular biology · 2015Article
- An optogenetic gene expression system with rapid activation and deactivation kinetics.Nature chemical biology · 2014Article
- Epstein-Barr virus induces global changes in cellular mRNA isoform usage that are important for the maintenance of latency.Journal of virology · 2013Article
- Structural insights into the targeting of mRNA GU-rich elements by the three RRMs of CELF1.Nucleic acids research · 2013Article
- Control of alternative splicing in immune responses: many regulators, many predictions, much still to learn.Immunological reviews · 2013Review
- Position-dependent and neuron-specific splicing regulation by the CELF family RNA-binding protein UNC-75 in Caenorhabditis elegans.Nucleic acids research · 2013Article
- Alternative Splicing of a Novel Inducible Exon Diversifies the CASK Guanylate Kinase Domain.Journal of nucleic acids · 2012Article
- Signal- and development-dependent alternative splicing of LEF1 in T cells is controlled by CELF2.Molecular and cellular biology · 2011Article
- Networking in a global world: establishing functional connections between neural splicing regulators and their target transcripts.RNA (New York, N.Y.) · 2011Review
- Regulation of alternative splicing by the core spliceosomal machinery.Genes & development · 2011Article
- Chromatin structure predicts epigenetic therapy responsiveness in sarcoma.Molecular cancer therapeutics · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Alternative pre-mRNA splicing adjusts the transcriptional output of the genome by generating related mRNAs from a single primary transcript, thereby expanding protein diversity. A fundamental unanswered question is how splicing factors achieve specificity in the selection of target substrates despite the recognition of information-poor sequence motifs. The CUGBP2 splicing regulator plays a key role in the brain region-specific silencing of the NI exon of the NMDA R1 receptor. However, the sequence motifs utilized by this factor for specific target exon selection and its role in splicing silencing are not understood. Here, we use chemical modification footprinting to map the contact sites of CUGBP2 to GU-rich motifs closely positioned at the boundaries of the branch sites of the NI exon, and we demonstrate a mechanistic role for this specific arrangement of motifs for the regulation of branchpoint formation. General support for a branch site-perimeter-binding model is indicated by the identification of a group of novel target exons with a similar configuration of motifs that are silenced by CUGBP2. These results reveal an autoregulatory role for CUGBP2 as indicated by its direct interaction with functionally significant RNA motifs surrounding the branch sites upstream of exon 6 of the CUGBP2 transcript itself. The perimeter-binding model explains how CUGBP2 can effectively embrace the branch site region to achieve the specificity needed for the selection of exon targets and the fine-tuning of alternative splicing patterns.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.