ArticleThe American journal of pathology2009
A novel organotypic model mimics the tumor microenvironment.
Article in The American journal of pathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 114 citations in OpenAlex.
- Quantitative and longitudinal monitoring of cancer cell invasion in a three-dimensional in vitro model of oral cancer using optical coherence tomography.Scientific reports · 2025Article
- Combining single-cell and bulk RNA sequencing to identify CAF-related signature for prognostic prediction and treatment response in patients with melanoma.Scientific reports · 2025Article
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- Novel human lymph node-derived matrix supports the adhesion of metastatic oral carcinoma cells.BMC cancer · 2023Article
- Stochastic differential equation modelling of cancer cell migration and tissue invasion.Journal of mathematical biology · 2023Article
- Stress induced phosphoprotein 1 overexpression controls proliferation, migration and invasion and is associated with poor survival in oral squamous cell carcinoma.Frontiers in oncology · 2022Article
- Bilberry (Antioxidants (Basel, Switzerland) · 2021Article
- Tumour cells express functional lymphatic endothelium-specific hyaluronan receptor in vitro and in vivo: Lymphatic mimicry promotes oral oncogenesis?Oncogenesis · 2021Article
- Three-Dimensional Cell Culture Models to Investigate Oral Carcinogenesis: A Scoping Review.International journal of molecular sciences · 2020Article
- Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells.International journal of molecular sciences · 2020Article
- Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells.Virology journal · 2020Article
- Novel anti-invasive properties of a Fascin1 inhibitor on colorectal cancer cells.Journal of molecular medicine (Berlin, Germany) · 2020Article
- Engineering approaches to studying cancer cell migration in three-dimensional environments.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- A Mathematical Framework for Modelling the Metastatic Spread of Cancer.Bulletin of mathematical biology · 2019Article
- Extracellular vesicles derived from cancer-associated fibroblasts induce the migration and invasion of oral squamous cell carcinoma.Journal of extracellular vesicles · 2019Article
- Organotypic three-dimensional assays based on human leiomyoma-derived matrices.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Three-dimensional organotypic matrices from alternative collagen sources as pre-clinical models for cell biology.Scientific reports · 2017Article
- The interplay of matrix metalloproteinase-8, transforming growth factor-β1 and vascular endothelial growth factor-C cooperatively contributes to the aggressiveness of oral tongue squamous cell carcinoma.British journal of cancer · 2017Article
- Fascin promotes migration and invasion and is a prognostic marker for oral squamous cell carcinoma.Oncotarget · 2017Article
- Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells inOncotarget · 2017Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carcinoma cell invasion is traditionally studied in three-dimensional organotypic models composed of type I collagen and fibroblasts. However, carcinoma cell behavior is affected by the various cell types and the extracellular matrix (ECM) in the tumor microenvironment. In this study, a novel organotypic model based on human uterine leiomyoma tissue was established and characterized to create a more authentic environment for carcinoma cells. Human tongue squamous cell carcinoma cells (HSC-3) were cultured on top of either collagen or myoma. Organotypic sections were examined by immunohistochemistry and in situ hybridization. The maximal invasion depth of HSC-3 cells was markedly increased in myomas compared with collagen. In myomas, various cell types and ECM components were present, and the HSC-3 cells only expressed ECM molecules in the myoma model. Organotypic media were analyzed by radioimmunoassay, zymography, or Western blotting. During carcinoma cell invasion, matrix metalloprotease-9 production and collagen degradation were enhanced particularly in the myoma model. To evaluate the general applicability of the myoma model, several oral carcinoma, breast carcinoma, and melanoma cell lines were cultured on myomas and found to invade in highly distinct patterns. We conclude that myoma tissue mimics the native tumor microenvironment better than previous organotypic models and possibly enhances epithelial-to-mesenchymal transition. Thus, the myoma model provides a promising tool for analyzing the behavior of carcinoma cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.