Evidence map›Paper›PMID 19679818›Full record

ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2009

P2Y5 is a G(alpha)i, G(alpha)12/13 G protein-coupled receptor activated by lysophosphatidic acid that reduces intestinal cell adhesion.

Mike Lee, Sungwon Choi, Gunnel Halldén, Sek Jin Yo, Denise Schichnes, Gregory W Aponte

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Gastrointestinal and liver physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 45 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. [Research progress on the biological regulatory function of lysophosphatidic acid in bone tissue cells].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2020
    Review
  8. Review
  9. Review
  10. Review
  11. Article
  12. Review
  13. Lysophosphatidic acid receptor, LPABiochemical and biophysical research communications · 2018
    Article
  14. Article
  15. Review
  16. Review
  17. Article
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Mike LeeDepartment of Nutritional Science and Toxicology, University of California at Berkeley, Berkeley, California 94720-3104, USA.
Sungwon Choi
Gunnel Halldén
Sek Jin Yo
Denise Schichnes
Gregory W Aponte
University of California, Berkeley · US

Funding

DIETARY FATTY ACID &EPITHELIAL CELL DIFFERENTIATIONR01DK058592 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI APONTE, GREGORY W. · 2001 to 2005
$1.2M
NIDDK NIH HHS DK58592
6 · The paper itself

Abstract

P2Y5 is a G protein-coupled receptor that binds and is activated by lysophosphatidic acid (LPA). We determined that P2Y5 transcript is expressed along the intestinal mucosa and investigated the intracellular pathways induced by P2Y5 activation, which could contribute to LPA effects on intestinal cell adhesion. P2Y5 heterologously expressed in CHO and small intestinal hBRIE 380i cells was activated by LPA resulting in an increase in intracellular calcium ([Ca(2+)](i)) when the cells concurrently expressed G(alpha)(Delta6qi5myr). P2Y5 activation also increased the phosphorylation of ERK1/2 that was sensitive to pertussis toxin. Together these indicate that P2Y5 activation by LPA induces an increase in [Ca(2+)](i) and ERK1/2 phosphorylation through G(alpha)(i). We discovered that P2Y5 was activated by farnesyl pyrophosphate (FPP) without a detectable change in [Ca(2+)](i). The activation of P2Y5 by LPA or FPP induced the activity of a serum response element (SRE)-linked luciferase reporter that was inhibited by the RGS domain of p115RhoGEF, C3 exotoxin, and Y-27632, suggesting the involvement of G(alpha)(12/13), Rho GTPase, and ROCK, respectively. However, only LPA-mediated induction of SRE reporter activity was sensitive to inhibitors targeting p38 MAPK, PI3K, PLC, and PKC. In addition, only LPA transactivated the epidermal growth factor receptor, leading to an induction of ERK1/2 phosphorylation. These observations correlate with our subsequent finding that P2Y5 activation by LPA, and not FPP, reduced intestinal cell adhesion. This study elucidates a mechanism whereby LPA can act as a luminal and/or serosal cue to alter mucosal integrity.

Indexed as

Cell AdhesionAnimalsCalcium SignalingCHO CellsCricetinaeCricetulusDown-RegulationEnzyme InhibitorsEpithelial CellsErbB ReceptorsG-Protein-Coupled Receptor Kinase 2GTP-Binding Protein alpha Subunits, G12-G13GTP-Binding Protein alpha Subunits, Gi-GoGuanine Nucleotide Exchange FactorsHeLa CellsHumansEnzyme InhibitorsErbB Receptorsfarnesyl pyrophosphateG-Protein-Coupled Receptor Kinase 2Grk2 protein, ratGTP-Binding Protein alpha Subunits, G12-G13GTP-Binding Protein alpha Subunits, Gi-GoGuanine Nucleotide Exchange Factorslysophosphatidic acidLysophospholipidsMAP Kinase Kinase 1MAP Kinase Kinase 2Mitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Polyisoprenyl PhosphatesReceptors, Purinergic P2Recombinant Fusion Proteinsrho-Associated Kinasesrho GTP-Binding ProteinsRho Guanine Nucleotide Exchange FactorsRNA, MessengerSesquiterpenes

Identifiers

PMID19679818
PMCPMC2763810
OpenAlexW2138548521

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.