ArticleThe Journal of biological chemistry2009
Cytomegalovirus promoter up-regulation is the major cause of increased protein levels of unstable reporter proteins after treatment of living cells with proteasome inhibitors.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Enhancement of activation-induced T cell proliferation by SIRPG in a CD47-independent manner.Frontiers in immunology · 2025Article
- Glycogen synthase kinase-3-mediated phosphorylation of serine 73 targets sterol response element binding protein-1c (SREBP-1c) for proteasomal degradation.Bioscience reports · 2015Article
- Calreticulin and Arginylated Calreticulin Have Different Susceptibilities to Proteasomal Degradation.The Journal of biological chemistry · 2015Article
- Human cytomegalovirus IE1 protein disrupts interleukin-6 signaling by sequestering STAT3 in the nucleus.Journal of virology · 2013Article
- Indirect inhibition of 26S proteasome activity in a cellular model of Huntington's disease.The Journal of cell biology · 2012Article
- A critical appraisal of quantitative studies of protein degradation in the framework of cellular proteostasis.Biochemistry research international · 2012Article
- Synphilin-1 inhibits alpha-synuclein degradation by the proteasome.Cellular and molecular life sciences : CMLS · 2011Article
- Quantitative cell-based protein degradation assays to identify and classify drugs that target the ubiquitin-proteasome system.The Journal of biological chemistry · 2011Article
- Phosphorylation regulates proteasomal-mediated degradation and solubility of TAR DNA binding protein-43 C-terminal fragments.Molecular neurodegeneration · 2010Article
- A photoconvertible reporter of the ubiquitin-proteasome system in vivo.Nature methods · 2010Article
- Acute polyglutamine expression in inducible mouse model unravels ubiquitin/proteasome system impairment and permanent recovery attributable to aggregate formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
Corrections and comments
- Retraction · 2016-04-22Duplication of/in Image · Euphemisms for Duplication · Manipulation of Images · Removed ·
- Retracted
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent unstable proteins obtained by the fusion of a fluorescent protein coding sequence with specific amino acid sequences that promote its fast degradation have become popular to gauge the activity of the ubiquitin/proteasome system in living cells. The steady-state levels of expression of these unstable proteins is low in agreement with their short half-lives, and they accumulate in the cell upon treatment with proteasome inhibitors. We show here that this accumulation is mainly due to transcriptional up-regulation of the cytomegalovirus promoter by proteasome inhibitors and mediated, at least in part, by AP1 transactivation. These simple facts put under quarantine conclusions reached about the activity of the ubiquitin/proteasome pathway in animal cells in culture or in transgenic mice, where popular cytomegalovirus-driven constructs are used, as transcriptional regulation of the expression of the reporter protein construct and not degradation of the unstable protein by the ubiquitin/proteasome system may contribute significantly to the interpretation of the results observed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.