ArticleThe Journal of biological chemistry2009
The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Karyopherin-mediated nucleocytoplasmic transport.Nature reviews. Molecular cell biology · 2022Review
- Biogenesis of RNA Polymerases in Yeast.Frontiers in molecular biosciences · 2021Review
- Article
- RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway.The New phytologist · 2016Article
- slc7a6os gene plays a critical role in defined areas of the developing CNS in zebrafish.PloS one · 2015Article
- Rtp1p is a karyopherin-like protein required for RNA polymerase II biogenesis.Molecular and cellular biology · 2013Article
- The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner.PLoS genetics · 2013Article
- The conserved foot domain of RNA pol II associates with proteins involved in transcriptional initiation and/or early elongation.Genetics · 2011Article
- Highly reproducible label free quantitative proteomic analysis of RNA polymerase complexes.Molecular & cellular proteomics : MCP · 2011Article
- Iwr1 protein is important for preinitiation complex formation by all three nuclear RNA polymerases in Saccharomyces cerevisiae.PloS one · 2011Article
- RNA-directed DNA methylation and plant development require an IWR1-type transcription factor.EMBO reports · 2010Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA polymerase II (RNA pol II) is a multisubunit enzyme that requires many auxiliary factors for its activity. Over the years, these factors have been identified using both biochemical and genetic approaches. Recently, the systematic characterization of protein complexes by tandem affinity purification and mass spectroscopy has allowed the identification of new components of well established complexes, including the RNA pol II holoenzyme. Using this approach, a novel and highly conserved factor, Iwr1p, that physically interacts with most of the RNA pol II subunits has been described in yeast. Here we show that Iwr1p genetically interacts with components of the basal transcription machinery and plays a role in both basal and regulated transcription. We report that mutation of the IWR1 gene is able to bypass the otherwise essential requirement for the transcriptional regulator negative cofactor 2, which occurs with different components of the basal transcription machinery, including TFIIA and subunits of the mediator complex. Deletion of the IWR1 gene leads to an altered expression of specific genes, including phosphate-responsive genes and SUC2. Our results show that Iwr1p is a nucleocytoplasmic shuttling protein and suggest that Iwr1p acts early in the formation of the pre-initiation complex by mediating the interaction of certain activators with the basal transcription apparatus.
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