ArticleDevelopmental biology2009
The cis-regulatory system of the tbrain gene: Alternative use of multiple modules to promote skeletogenic expression in the sea urchin embryo.
Article in Developmental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Single-cell transcriptomics reveals evolutionary reconfiguration of embryonic cell fate specification in the sea urchinbioRxiv : the preprint server for biology · 2024Article
- Cell type phylogenetics informs the evolutionary origin of echinoderm larval skeletogenic cell identity.Communications biology · 2019Article
- Developmental effector gene regulation: Multiplexed strategies for functional analysis.Developmental biology · 2019Article
- From genome to anatomy: The architecture and evolution of the skeletogenic gene regulatory network of sea urchins and other echinoderms.Genesis (New York, N.Y. : 2000) · 2018Review
- Bacterial artificial chromosomes as recombinant reporter constructs to investigate gene expression and regulation in echinoderms.Briefings in functional genomics · 2018Review
- Global analysis of primary mesenchyme cell cis-regulatory modules by chromatin accessibility profiling.BMC genomics · 2018Article
- Evolutionary rewiring of gene regulatory network linkages at divergence of the echinoid subclasses.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- microRNAs regulate β-catenin of the Wnt signaling pathway in early sea urchin development.Developmental biology · 2015Article
- General approach for in vivo recovery of cell type-specific effector gene sets.Genome research · 2014Article
- An enhancer composed of interlocking submodules controls transcriptional autoregulation of suppressor of hairless.Developmental cell · 2014Article
- Experimental approaches for gene regulatory network construction: the chick as a model system.Genesis (New York, N.Y. : 2000) · 2013Review
- Synthetic in vivo validation of gene network circuitry.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Precise cis-regulatory control of spatial and temporal expression of the alx-1 gene in the skeletogenic lineage of s. purpuratus.Developmental biology · 2011Article
- Evolutionary crossroads in developmental biology: sea urchins.Development (Cambridge, England) · 2011Review
- Information processing at the foxa node of the sea urchin endomesoderm specification network.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Functional cis-regulatory genomics for systems biology.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Regulative recovery in the sea urchin embryo and the stabilizing role of fail-safe gene network wiring.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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5 authors.
Funding
Abstract
The genomic cis-regulatory systems controlling regulatory gene expression usually include multiple modules. The regulatory output of such systems at any given time depends on which module is directing the function of the basal transcription apparatus, and ultimately on the transcription factor inputs into that module. Here we examine regulation of the Strongylocentrotus purpuratus tbrain gene, a required activator of the skeletogenic specification state in the lineage descendant from the embryo micromeres. Alternate cis-regulatory modules were found to convey skeletogenic expression in reporter constructs. To determine their relative developmental functions in context, we made use of recombineered BAC constructs containing a GFP reporter and of derivatives from which specific modules had been deleted. The outputs of the various constructs were observed spatially by GFP fluorescence and quantitatively over time by QPCR. In the context of the complete genomic locus, early skeletogenic expression is controlled by an intron enhancer plus a proximal region containing a HesC site as predicted from network analysis. From ingression onward, however, a dedicated distal module utilizing positive Ets1/2 inputs contributes to definitive expression in the skeletogenic mesenchyme. This module also mediates a newly discovered negative Erg input which excludes non-skeletogenic mesodermal expression.
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