ArticleFEMS immunology and medical microbiology2009
Use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters in the Vibrio cholerae vaccine strain CVD 103-HgR.
Article in FEMS immunology and medical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
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- Evaluation of Three Candidate Live-AttenuatedVaccines · 2023Article
- Engineered Escherichia coli for the in situ secretion of therapeutic nanobodies in the gut.Cell host & microbe · 2023Article
- A Strategy for the Rapid Development of a SafeInternational journal of molecular sciences · 2021Article
- Uropathogenic E. coli induces DNA damage in the bladder.PLoS pathogens · 2021Article
- The Genotoxin Colibactin Shapes Gut Microbiota in Mice.mSphere · 2020Article
- Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System.Journal of visualized experiments : JoVE · 2018Article
- The Vibrio cholerae RND efflux systems impact virulence factor production and adaptive responses via periplasmic sensor proteins.PLoS pathogens · 2018Article
- Murine immunization with CS21 pili or LngA major subunit of enterotoxigenic Escherichia coli (ETEC) elicits systemic and mucosal immune responses and inhibits ETEC gut colonization.Veterinary microbiology · 2017Article
- Vibrio cholerae leuO Transcription Is Positively Regulated by ToxR and Contributes to Bile Resistance.Journal of bacteriology · 2015Article
- Expression of enteropathogenic Escherichia coli map is significantly different than that of other type III secreted effectors in vivo.Infection and immunity · 2015Article
- Substrate-dependent activation of the Vibrio cholerae vexAB RND efflux system requires vexR.PloS one · 2015Article
- Construction of a tetracycline inducible expression vector and characterization of its use in Vibrio cholerae.Plasmid · 2014Article
- Vibrio cholerae ToxR downregulates virulence factor production in response to cyclo(Phe-Pro).mBio · 2013Article
- Development of a Fur-dependent and tightly regulated expression system in Escherichia coli for toxic protein synthesis.BMC biotechnology · 2013Article
- Additive function of Vibrio vulnificus MARTX(Vv) and VvhA cytolysins promotes rapid growth and epithelial tissue necrosis during intestinal infection.PLoS pathogens · 2012Article
- Iron-regulated lysis of recombinant Escherichia coli in host releases protective antigen and confers biological containment.Infection and immunity · 2011Article
- Enterohemorrhagic E. coli alters murine intestinal epithelial tight junction protein expression and barrier function in a Shiga toxin independent manner.Laboratory investigation; a journal of technical methods and pathology · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Live, attenuated Vibrio cholerae vaccines can induce potent immune responses after only a single oral dose. The strategy of harnessing these strains to present antigens from heterologous pathogens to the mucosal immune system shows great promise. To fully realize this possibility, V. cholerae strains must be created that stably express antigens in vivo in sufficient quantity to generate an immune response. In vivo-induced promoters have been shown to increase the stability and immunogenicity of foreign antigens expressed from multicopy plasmids. We report the construction of a series of genetically stabilized plasmids expressing luciferase as a heterologous protein from the following in vivo-induced promoters: V. cholerae P(argC), P(fhuC) and P(vca1008), and Salmonella enterica serovar Typhi P(ompC). We demonstrate that several of these expression plasmids meet two critical criteria for V. cholerae live vector vaccine studies. First, the plasmids are highly stable in the V. cholerae vaccine strain CVD 103-HgR at low copy number, in the absence of selective pressure. Second, real-time bioluminescent imaging (BLI) demonstrates inducible in vivo expression of the promoters in the suckling mouse model of V. cholerae colonization. Moreover, the use of BLI allows for direct quantitative comparison of in vivo expression from four different promoters at various time points.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.