ArticleThe Journal of physiology2009
Kif5b is an essential forward trafficking motor for the Kv1.5 cardiac potassium channel.
Article in The Journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Microtubule plus-end tracking proteins: novel modulators of cardiac sodium channels and arrhythmogenesis.Cardiovascular research · 2023Article
- Actin-microtubule cytoskeletal interplay mediated by MRTF-A/SRF signaling promotes dilated cardiomyopathy caused by LMNA mutations.Nature communications · 2022Article
- Cardiomyocyte Microtubules: Control of Mechanics, Transport, and Remodeling.Annual review of physiology · 2022Review
- Oxidative stress pathogenically remodels the cardiac myocyte cytoskeleton via structural alterations to the microtubule lattice.Developmental cell · 2021Article
- Kv7 channel trafficking by the microtubule network in vascular smooth muscle.Acta physiologica (Oxford, England) · 2021Review
- Fluvastatin inhibits Rab5-mediated IKs internalization caused by chronic CaJournal of molecular and cellular cardiology · 2019Article
- A Germline Variant in the PANX1 Gene Has Reduced Channel Function and Is Associated with Multisystem Dysfunction.The Journal of biological chemistry · 2016Article
- Oxidative stress decreases microtubule growth and stability in ventricular myocytes.Journal of molecular and cellular cardiology · 2016Article
- Identification of Kinesin-1 Cargos Using Fluorescence Microscopy.Journal of visualized experiments : JoVE · 2016Article
- Transitions of protein traffic from cardiac ER to junctional SR.Journal of molecular and cellular cardiology · 2015Article
- Microtubule-mediated defects in junctophilin-2 trafficking contribute to myocyte transverse-tubule remodeling and Ca2+ handling dysfunction in heart failure.Circulation · 2014Article
- Role for myosin-V motor proteins in the selective delivery of Kv channel isoforms to the membrane surface of cardiac myocytes.Circulation research · 2014Article
- KIF5B promotes the forward transport and axonal function of the voltage-gated sodium channel Nav1.8.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- L-type calcium channel targeting and local signalling in cardiac myocytes.Cardiovascular research · 2013Review
- Multilayered regulation of cardiac ion channels.Biochimica et biophysica acta · 2013Review
- Remodeling of mechanical junctions and of microtubule-associated proteins accompany cardiac connexin43 lateralization.Heart rhythm · 2012Article
- Function and mechanism of axonal targeting of voltage-sensitive potassium channels.Progress in neurobiology · 2011Review
- Dynamic changes in sarcoplasmic reticulum structure in ventricular myocytes.Journal of biomedicine & biotechnology · 2011Article
- Kinesin I transports tetramerized Kv3 channels through the axon initial segment via direct binding.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Emerging concepts in the pharmacogenomics of arrhythmias: ion channel trafficking.Expert review of cardiovascular therapy · 2010Review
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8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the role of the kinesin I isoform Kif5b in the trafficking of a cardiac voltage-gated potassium channel, Kv1.5. In Kv1.5-expressing HEK293 cells and H9c2 cardiomyoblasts, current densities were increased from control levels of 389 +/- 50.0 and 317 +/- 50.3 pA pF(1), respectively, to 614 +/- 74.3 and 580 +/- 90.9 pA pF(1) in cells overexpressing the Kif5b motor. Overexpression of the Kif5b motor increased Kv1.5 expression additively with several manipulations that reduce channel internalization, suggesting that it is involved in the delivery of the channel to the cell surface. In contrast, expression of a Kif5b dominant negative (Kif5bDN) construct increased Kv1.5 expression non-additively with these manipulations. Thus, the dominant negative acts by indirectly inhibiting endocytosis. The increase in Kv1.5 currents induced by wild-type Kif5b was dependent on Golgi function; a 6 h treatment with Brefeldin A reduced Kv1.5 currents to control levels in Kif5b-overexpressing cells but had little effect on the increase associated with Kif5bDN expression. Finally, expression of the Kif5bDN prior to induction of Kv1.5 in a tetracycline inducible system blocked surface expression of the channel in both HEK293 cells and H9c2 cardiomyoblasts. Thus, Kif5b is essential to anterograde trafficking of a cardiac voltage-gated potassium channel.
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