ArticleProceedings of the National Academy of Sciences of the United States of America1990
Molecular dissection of mutations at the heterozygous thymidine kinase locus in mouse lymphoma cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 149 citations in OpenAlex.
- In Vitro Mouse Lymphoma Cell (L5178Y TkMethods in molecular biology (Clifton, N.J.) · 2026Article
- Key Challenges forAlternatives to laboratory animals : ATLA · 2024Review
- Thymidine KinaseFrontiers in toxicology · 2022Article
- Detection of Loss of Heterozygosity in Tk-Deficient Mutants from L5178Y TkMethods in molecular biology (Clifton, N.J.) · 2020Article
- Quantitative analysis of the relative mutagenicity of five chemical constituents of tobacco smoke in the mouse lymphoma assay.Mutagenesis · 2016Article
- Article
- Silver nanoparticle-induced mutations and oxidative stress in mouse lymphoma cells.Environmental and molecular mutagenesis · 2012Article
- Mutagenicity of 11 cigarette smoke condensates in two versions of the mouse lymphoma assay.Mutagenesis · 2011Article
- Cytotoxicity and mutagenicity of retinol with ultraviolet A irradiation in mouse lymphoma cells.Toxicology in vitro : an international journal published in association with BIBRA · 2010Article
- Integration of in vivo and in vitro approaches to characterize the toxicity of Antalarmin, a corticotropin-releasing hormone receptor antagonist.Toxicology · 2008Article
- Genotoxic effects of acrylamide and glycidamide in mouse lymphoma cells.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2008Article
- Photomutagenicity of anhydroretinol and 5,6-epoxyretinyl palmitate in mouse lymphoma cells.Chemical research in toxicology · 2006Article
- Microarray analysis distinguishes differential gene expression patterns from large and small colony Thymidine kinase mutants of L5178Y mouse lymphoma cells.BMC bioinformatics · 2006Article
- Photomutagenicity of retinyl palmitate by ultraviolet a irradiation in mouse lymphoma cells.Toxicological sciences : an official journal of the Society of Toxicology · 2005Article
- Mutagenicity of trichloroethylene and its metabolites: implications for the risk assessment of trichloroethylene.Environmental health perspectives · 2000Review
- Relation of the slow growth phenotype to neoplastic transformation: possible significance for human cancer.In vitro cellular & developmental biology. Animal · 1999Article
- Carcinogen-induced loss of heterozygosity at the Aprt locus in somatic cells of the mouse.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Different capacities for recombination in closely related human lymphoblastoid cell lines with different mutational responses to X-irradiation.Molecular and cellular biology · 1994Article
- Nuclear localization of the ubiquitin-activating enzyme, E1, is cell-cycle-dependent.The Biochemical journal · 1994Article
- Quantification and molecular characterization of hprt mutants of human T-lymphocytes.Environmental health perspectives · 1993Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mouse lymphoma L5178Y TK+/- 3.7.2C cell line allows quantitation of induced TK(+/-)----TK-/- mutations at the heterozygous thymidine kinase (Tk) locus. TK-/- mutant colonies show a bimodal size distribution, reflecting a difference in the growth rates of the two size classes that is hypothesized to result from different degrees of genetic damage. The two homologous chromosomes 11 containing the alleles of the Tk gene in L5178Y 3.7.2C TK+/- cells are distinguishable at the cytogenetic level. We find, in addition, that the two alleles are distinguishable at the molecular level because of an Nco I restriction fragment length polymorphism at the 3' end of the gene. In a set of 51 large-colony and 48 small-colony TK-/- mutants induced by ionizing radiation or by chemical mutagens, we find that 78, including all except one of the small-colony mutants, have lost the Tk+ allele and that some of these have two to four copies of the remaining Tk- allele. Nineteen of the large-colony TK-/- mutants that do not show Tk+ allele loss show no other structural changes detectable at the level of Southern blot analysis. One shows a partial deletion. The variety of mutagenic lesions recorded at the heterozygous Tk locus may be representative of events observed in human malignancy and may include a wider range of mutagenic events than can be observed at hemizygous test loci.
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