ArticleAlcoholism, clinical and experimental research2009
Ethanol inhibits muscarinic receptor-induced axonal growth in rat hippocampal neurons.
Article in Alcoholism, clinical and experimental research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Assessment of ethanol exposure from hand sanitizer use and potential for developmental toxicity in nursing infants.Journal of applied toxicology : JAT · 2022Review
- Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum.International journal of molecular sciences · 2021Article
- M1 muscarinic receptor is a key target of neuroprotection, neuroregeneration and memory recovery by i-Extract from Withania somnifera.Scientific reports · 2019Article
- Role of Thioredoxin 1 in Impaired Renal Sodium Excretion of hDJournal of the American Heart Association · 2019Article
- Neonatal Ethanol and Choline Treatments Alter the Morphology of Developing Rat Hippocampal Pyramidal Neurons in Opposite Directions.Neuroscience · 2018Article
- Ethanol-induced changes in poly (ADP ribose) polymerase and neuronal developmental gene expression.Neuropharmacology · 2016Article
- Developmental alcohol exposure leads to a persistent change on astrocyte secretome.Journal of neurochemistry · 2016Article
- Hippocampal neuron populations are reduced in vervet monkeys with fetal alcohol exposure.Developmental psychobiology · 2015Article
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- Astrocytes protect against diazinon- and diazoxon-induced inhibition of neurite outgrowth by regulating neuronal glutathione.Toxicology · 2014Article
- Diazinon and diazoxon impair the ability of astrocytes to foster neurite outgrowth in primary hippocampal neurons.Toxicology and applied pharmacology · 2014Article
- Ethanol affects network activity in cultured rat hippocampus: mediation by potassium channels.PloS one · 2013Article
- Long-term consequences of developmental alcohol exposure on brain structure and function: therapeutic benefits of physical activity.Brain sciences · 2012Article
- Genistein inhibits Aβ₂₅₋₃₅ -induced neurotoxicity in PC12 cells via PKC signaling pathway.Neurochemical research · 2012Article
- Ethanol impairs muscarinic receptor-induced neuritogenesis in rat hippocampal slices: Role of astrocytes and extracellular matrix proteins.Biochemical pharmacology · 2011Article
- Ethanol differentially regulates snail family of transcription factors and invasion of premalignant and malignant pancreatic ductal cells.Journal of cellular biochemistry · 2011Article
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- Prenatal choline supplementation mitigates behavioral alterations associated with prenatal alcohol exposure in rats.Birth defects research. Part A, Clinical and molecular teratology · 2010Article
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4 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundIn utero alcohol exposure can lead to fetal alcohol spectrum (FAS) disorders characterized by cognitive and behavioral deficits. In vivo and in vitro studies have shown that ethanol alters neuronal development. One mechanism through which ethanol has been shown to exert its effects is the perturbation of activated signaling cascades. The cholinergic agonist carbachol has been shown to induce axonal outgrowth through intracellular calcium mobilization, protein kinase C (PKC) activation, and ERK1/2 phosphorylation. This study investigated the effect of ethanol on the differentiation of rat hippocampal pyramidal neurons induced by carbachol as a possible mechanism involved in the developmental neurotoxicity of ethanol.
methodsPrenatal rat hippocampal pyramidal neurons were treated with ethanol (50 to 75 mM) in the presence or absence of carbachol for 24 hours. Neurite outgrowth was assessed spectrophotometrically; axonal length was measured in neurons fixed and immunolabeled with the neuron-specific betaIII tubulin antibody; cytotoxicity was analyzed using the thiazolyl blue tetrazolium bromide assay. The effect of ethanol on carbachol-stimulated intracellular calcium mobilization was assessed utilizing the fluorescent calcium probe, Fluo-3AM. The PepTag(R) assay for nonradioactive detection of PKC from Promega was used to measure PKC activity, and ERK1/2 activation was determined by densitometric analysis of Western blots probed for phospo-ERK1/2.
resultsEthanol treatment (50 to 75 mM) caused an inhibition of carbachol-induced axonal growth, without affecting neuronal viability. Neuron treatment for 15 minutes with ethanol did not inhibit the carbachol-stimulated rise in intracellular calcium, while inhibiting PKC activity at the highest tested concentration and ERK1/2 phosphorylation at both the concentrations used in this study. On the other hand, neuron treatment for 24 hours with ethanol significantly inhibited carbachol-induced increase in intracellular calcium.
conclusionsEthanol inhibited carbachol-induced neurite outgrowth by inhibiting PKC and ERK1/2 activation. These effects may be, in part, responsible for some of the cognitive deficits associated with in utero alcohol exposure.
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