ArticleThe Journal of comparative neurology2009
Viral strategies for studying the brain, including a replication-restricted self-amplifying delta-G vesicular stomatis virus that rapidly expresses transgenes in brain and can generate a multicolor golgi-like expression.
Article in The Journal of comparative neurology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 52 citations in OpenAlex.
- Unraveling the Neural Circuits: Techniques, Opportunities and Challenges in Epilepsy Research.Cellular and molecular neurobiology · 2024Review
- Synaptic configuration and reconfiguration in the neocortex are spatiotemporally selective.Anatomical science international · 2024Review
- Article
- Molecular profiling of enteric nervous system cell lineages.Nature protocols · 2022Review
- Illuminating Neural Circuits in Alzheimer's Disease.Neuroscience bulletin · 2021Review
- Viral tools for neuroscience.Nature reviews. Neuroscience · 2020Review
- Bioactive Natural Antivirals: An Updated Review of the Available Plants and Isolated Molecules.Molecules (Basel, Switzerland) · 2020Review
- A mutant vesicular stomatitis virus with reduced cytotoxicity and enhanced anterograde trans-synaptic efficiency.Molecular brain · 2020Article
- Trans-synaptic Neural Circuit-Tracing with Neurotropic Viruses.Neuroscience bulletin · 2019Review
- Hitchhiking on the neuronal highway: Mechanisms of transsynaptic specificity.Journal of chemical neuroanatomy · 2019Review
- Development of an Acute Method to Deliver Transgenes Into the Brains of AdultFrontiers in neural circuits · 2018Article
- Review
- Chikungunya, Influenza, Nipah, and Semliki Forest Chimeric Viruses with Vesicular Stomatitis Virus: Actions in the Brain.Journal of virology · 2017Article
- Vesicular stomatitis virus enables gene transfer and transsynaptic tracing in a wide range of organisms.The Journal of comparative neurology · 2015Article
- Attenuation of vesicular stomatitis virus infection of brain using antiviral drugs and an adeno-associated virus-interferon vector.Virology · 2015Article
- An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology.Brain structure & function · 2015Article
- Neuroanatomy goes viral!Frontiers in neuroanatomy · 2015Review
- Controlling feeding behavior by chemical or gene-directed targeting in the brain: what's so spatial about our methods?Frontiers in neuroscience · 2013Review
- Synthesis and properties of Asante Calcium Red--a novel family of long excitation wavelength calcium indicators.Cell calcium · 2013Article
- Highly attenuated recombinant vesicular stomatitis virus VSV-12'GFP displays immunogenic and oncolytic activity.Journal of virology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Viruses have substantial value as vehicles for transporting transgenes into neurons. Each virus has its own set of attributes for addressing neuroscience-related questions. Here we review some of the advantages and limitations of herpes, pseudorabies, rabies, adeno-associated, lentivirus, and others to study the brain. We then explore a novel recombinant vesicular stomatitis virus (dG-VSV) with the G-gene deleted and transgenes engineered into the first position of the RNA genome, which replicates only in the first brain cell infected, as corroborated with ultrastructural analysis, eliminating spread of virus. Because of its ability to replicate rapidly and to express multiple mRNA copies and additional templates for more copies, reporter gene expression is amplified substantially, over 500-fold in 6 hours, allowing detailed imaging of dendrites, dendritic spines, axons, and axon terminal fields within a few hours to a few days after inoculation. Green fluorescent protein (GFP) expression is first detected within 1 hour of inoculation. The virus generates a Golgi-like appearance in all neurons or glia of regions of the brain tested. Whole-cell patch-clamp electrophysiology, calcium digital imaging with fura-2, and time-lapse digital imaging showed that neurons appeared physiologically normal after expressing viral transgenes. The virus has a wide range of species applicability, including mouse, rat, hamster, human, and Drosophila cells. By using dG-VSV, we show efferent projections from the suprachiasmatic nucleus terminating in the periventricular region immediately dorsal to the nucleus. DG-VSVs with genes coding for different color reporters allow multicolor visualization of neurons wherever applied.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.