ArticlePloS one2009
In vitro and in vivo evidence that thrombospondin-1 (TSP-1) contributes to stirring- and shear-dependent activation of platelet-derived TGF-beta1.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 51 citations in OpenAlex.
- Identification of the thiol isomerase-binding peptide, mastoparan, as a novel inhibitor of shear-induced transforming growth factor β1 (TGF-β1) activation.The Journal of biological chemistry · 2013Trial
- Comparing Heparin and Heparin Mimetics in Targeting Immunomodulatory Proteins from Platelets to Activate T Cell-Dependent Immune Response in Oncology.ACS pharmacology & translational science · 2026Article
- MacrophageJournal for immunotherapy of cancer · 2026Article
- Inflammation identity crisis: pathogenic nature of platelet-derived transforming growth factor β in chronic inflammatory conditions.Frontiers in immunology · 2026Review
- N-Acetylcysteine Inhibits Aortic Stenosis Progression in a Murine Model by Blocking Shear-Induced Activation of Platelet Latent Transforming Growth Factor Beta 1.Antioxidants & redox signaling · 2024Article
- A possible role of plasmin-dependent activation of TGF-β in cancer-associated thrombosis: Implications for therapy.Cancer metastasis reviews · 2024Review
- Stromal thrombospondin 1 suppresses angiogenesis in oral submucous fibrosis.International journal of oral science · 2024Article
- Scleral remodeling during myopia development in mice eyes: a potential role of thrombospondin-1.Molecular medicine (Cambridge, Mass.) · 2024Article
- Mechanical regulation of chromatin and transcription.Nature reviews. Genetics · 2022Review
- Angiogenic content of microparticles in patients with diabetes and coronary artery disease predicts networks of endothelial dysfunction.Cardiovascular diabetology · 2022Article
- Stem cell-derived and circulating exosomal microRNAs as new potential tools for diabetic nephropathy management.Stem cell research & therapy · 2022Review
- Osteoimmunomodulation role of exosomes derived from immune cells on osseointegration.Frontiers in bioengineering and biotechnology · 2022Review
- Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion.Redox biology · 2021Article
- RUNX-1 haploinsufficiency causes a marked deficiency of megakaryocyte-biased hematopoietic progenitor cells.Blood · 2021Article
- Blood Platelets as an Important but Underrated Circulating Source of TGFβ.International journal of molecular sciences · 2021Review
- Idiopathic Pulmonary Comorbidities and Mechanisms.International journal of inflammation · 2021Review
- Platelet TSP-1 controls prostate cancer-induced osteoclast differentiation and bone marrow-derived cell mobilization through TGFβ-1.American journal of clinical and experimental urology · 2021Article
- Quantitative planar array screen of 1000 proteins uncovers novel urinary protein biomarkers of lupus nephritis.Annals of the rheumatic diseases · 2020Article
- Role of thrombospondin‑1 and thrombospondin‑2 in cardiovascular diseases (Review).International journal of molecular medicine · 2020Review
- Oscillatory shear potentiates latent TGF-β1 activation more than steady shear as demonstrated by a novel force generator.Scientific reports · 2019Observational
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Thrombospondin 1 (TSP-1), which is contained in platelet alpha-granules and released with activation, has been shown to activate latent TGF-beta1 in vitro, but its in vivo role is unclear as TSP-1-null (Thbs1(-/-)) mice have a much less severe phenotype than TGF-beta1-null (Tgfb1(-/-)) mice. We recently demonstrated that stirring and/or shear could activate latent TGF-beta1 released from platelets and have now studied these methods of TGF-beta1 activation in samples from Thbs1(-/-) mice, which have higher platelet counts and higher levels of total TGF-beta1 in their serum than wild type mice. After either two hours of stirring or shear, Thbs1(-/-) samples demonstrated less TGF-beta1 activation (31% and 54% lower levels of active TGF-beta1 in serum and platelet releasates, respectively). TGF-beta1 activation in Thbs1(-/-) mice samples was normalized by adding recombinant human TSP-1 (rhTSP-1). Exposure of platelet releasates to shear for one hour led to near depletion of TSP-1, but this could be prevented by preincubating samples with thiol-reactive agents. Moreover, replenishing rhTSP-1 to human platelet releasates after one hour of stirring enhanced TGF-beta1 activation. In vivo TGF-beta1 activation in carotid artery thrombi was also partially impaired in Thbs1(-/-) mice. These data indicate that TSP-1 contributes to shear-dependent TGF-beta1 activation, thus providing a potential explanation for the inconsistent in vitro data previously reported as well as for the differences in phenotypes of Thbs1(-/-) and Tgfb1(-/-) mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.