ArticleMolecular human reproduction2009
Evaluation of genome coverage and fidelity of multiple displacement amplification from single cells by SNP array.
Article in Molecular human reproduction, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Accurate and scalable variant calling from single cell DNA sequencing data with ProSolo.Nature communications · 2021Article
- Preimplantation Genetic Diagnosis for DEB by Detecting a Novel Family-Specific COL7A1 Mutation in Vietnam.The application of clinical genetics · 2021Article
- Systematic assessment of the performance of whole-genome amplification for SNP/CNV detection and β-thalassemia genotyping.Journal of human genetics · 2018Article
- Comparison of methods for library construction and short read annotation of shellfish viral metagenomes.Genes & genomics · 2018Article
- Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates.BMC genomics · 2014Article
- Reliable single cell array CGH for clinical samples.PloS one · 2014Article
- SNP array-based copy number and genotype analyses for preimplantation genetic diagnosis of human unbalanced translocations.European journal of human genetics : EJHG · 2012Article
- Isolation of Plasmodium falciparum by flow-cytometry: implications for single-trophozoite genotyping and parasite DNA purification for whole-genome high-throughput sequencing of archival samples.Malaria journal · 2012Article
- Methods for comprehensive chromosome screening of oocytes and embryos: capabilities, limitations, and evidence of validity.Journal of assisted reproduction and genetics · 2012Review
- Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor.Cell · 2012Article
- An update of preimplantation genetic diagnosis in gene diseases, chromosomal translocation, and aneuploidy screening.Clinical and experimental reproductive medicine · 2011Article
- Laser capture microdissection (LCM) and whole genome amplification (WGA) of DNA from normal breast tissue --- optimization for genome wide array analyses.BMC research notes · 2011Article
- Whole genome amplification in preimplantation genetic diagnosis.Journal of Zhejiang University. Science. B · 2011Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The scarce amount of DNA contained in a single cell is a limiting factor for clinical application of preimplantation genetic diagnosis mainly due to the risk of misdiagnosis caused by allele dropout and the difficulty in obtaining copy number variations in all 23 pairs of chromosomes. Multiple displacement amplification (MDA) has been reported to generate large quantity of products from small amount of templates. Here, we evaluated the fidelity of whole-genome amplification MDA from single or a few cells and determined the accuracy of chromosome copy number assessment on these MDA products using an Affymetrix 10K 2.0 SNP Mapping Array. An average coverage rate (86.2%) from single cells was obtained and the rates increased significantly when five or more cells were used as templates. Higher concordance for chromosome copy number from single cells could be achieved when the MDA amplified product was used as reference (93.1%) than when gDNA used as reference (82.8%). The present study indicates that satisfactory genome coverage can be obtained from single-cell MDA which may be used for studies where only a minute amount of genetic materials is available. Clinically, MDA coupled with SNP mapping array may provide a reliable and accurate method for chromosome copy number analysis and most likely for the detection of single-gene disorders as well.
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