ArticleGeochemical transactions2009
Evaluating the use of 3'-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions.
Article in Geochemical transactions, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed.
- ROS-responsive drug delivery systems for chronic wound healing: advances, challenges, and translational perspectives.Military Medical Research · 2026Review
- Nanobubble Reactivity: Evaluating Hydroxyl Radical Generation (or Lack Thereof) under Ambient Conditions.ACS ES&T engineering · 2023Article
- Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets.ACS catalysis · 2023Article
- Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques.Pharmaceutics · 2021Review
- Oxidative stress decreases the redox ratio and folate content in the gut microbe, Enterococcus durans (MTCC 3031).Scientific reports · 2018Article
- The Role of Iraqi Dust in Inducing Lung Injury in United States Soldiers-An Interdisciplinary Study.GeoHealth · 2017Article
- A Novel High-Throughput Approach to Measure Hydroxyl Radicals Induced by Airborne Particulate Matter.International journal of environmental research and public health · 2015Article
- The effect of pyrite on Escherichia coli in water: proof-of-concept for the elimination of waterborne bacteria by reactive minerals.Journal of water and health · 2015Article
- Metal-sulfide mineral ores, Fenton chemistry and disease--particle induced inflammatory stress response in lung cells.International journal of hygiene and environmental health · 2015Article
- Pyrite-driven reactive oxygen species formation in simulated lung fluid: implications for coal workers' pneumoconiosis.Environmental geochemistry and health · 2012Article
- Did mineral surface chemistry and toxicity contribute to evolution of microbial extracellular polymeric substances?Astrobiology · 2012Article
- Phenylalanine as a hydroxyl radical-specific probe in pyrite slurries.Geochemical transactions · 2012Article
- Environmentally persistent free radicals (EPFRs)-2. Are free hydroxyl radicals generated in aqueous solutions?Environmental science & technology · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGiven the importance of highly reactive oxygen species (hROS) as reactants in a wide range of biological, photochemical, and environmental systems there is an interest in detection and quantification of these species. The extreme reactivity of the hROS, which includes hydroxyl radicals, presents an analytical challenge. 3'-(p-Aminophenyl) fluorescein (APF) is a relatively new probe used for measuring hROS. Here, we further evaluate the use of APF as a method for the detection of hydroxyl radicals in particle suspensions.
resultsParticle-generated hROS can be quantified with an estimated detection limit of 50 nM. Measurements of hROS in two National Institute of Standards and Technology (NIST 2709 and 2710) soil suspensions and a pyrite suspension show non-linear particle dose-response curves for hROS generation. APF can also be used in solutions containing no dissolved molecular oxygen (O2) to determine the role of O2 in the formation of hROS. Results confirm that O2 is mechanistically important in the formation of hROS by dissolved ferrous iron and in pyrite suspensions.
conclusionGiven the non-linear dose-response curves for particle generation of hROS, we recommend using several particle loadings in experiments aimed to compare particles for their hROS generation potential. The method presented here is specific to hROS and simple to perform. The analysis can be conducted in mobile labs as only basic laboratory equipment is required.
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