ArticleBiochemistry2009
Mapping the interaction of pro-apoptotic tBID with pro-survival BCL-XL.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- The BCL2 family: from apoptosis mechanisms to new advances in targeted therapy.Signal transduction and targeted therapy · 2025Review
- Bid Protein: A Participant in the Apoptotic Network with Roles in Viral Infections.International journal of molecular sciences · 2025Review
- Apoptotic mitochondrial poration by a growing list of pore-forming BCL-2 family proteins.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- Conformational States of the Cytoprotective Protein Bcl-xL.Biophysical journal · 2020Article
- Lipoprotein Particle Formation by Proapoptotic tBid.Biophysical journal · 2018Article
- Regulation of apoptosis by an intrinsically disordered region of Bcl-xL.Nature chemical biology · 2018Article
- The Bcl-2 Family in Host-Virus Interactions.Viruses · 2017Review
- Dynamic Protein Interaction Networks and New Structural Paradigms in Signaling.Chemical reviews · 2016Review
- Conformation of BCL-XL upon Membrane Integration.Journal of molecular biology · 2015Article
- Bid chimeras indicate that most BH3-only proteins can directly activate Bak and Bax, and show no preference for Bak versus Bax.Cell death & disease · 2015Article
- Molecular determinants of the binding specificity of BH3 ligands to BclXL apoptotic repressor.Biopolymers · 2014Article
- Detergent optimized membrane protein reconstitution in liposomes for solid state NMR.Biochemistry · 2014Article
- Structural insights of tBid, the caspase-8-activated Bid, and its BH3 domain.The Journal of biological chemistry · 2013Article
- Structural biology of the Bcl-2 family and its mimicry by viral proteins.Cell death & disease · 2013Review
- Biophysical basis of the promiscuous binding of B-cell lymphoma protein 2 apoptotic repressor to BH3 ligands.Journal of molecular recognition : JMR · 2013Article
- Apoptotic regulation by MCL-1 through heterodimerization.The Journal of biological chemistry · 2010Article
- Intrinsically disordered proteins in bcl-2 regulated apoptosis.International journal of molecular sciences · 2010Review
- BAX and BAK caught in the act.Molecular cell · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The BH3-only BCL-2 family protein BID is activated by caspase-8 cleavage upon engagement of cell surface death receptors. The resulting 15 kDa C-terminal fragment, tBID, translocates to mitochondria, triggering the release of cytotoxic molecules and cell death. The pro-apoptotic activity of tBID is regulated by its interactions with pro-survival BCL-XL and pro-death BAX, both in the cytosol and at the mitochondrial membrane. In this study, we characterize the molecular interactions between full-length tBID and BCL-XL using NMR spectroscopy and isothermal titration calorimetry (ITC). In aqueous solution, tBID adopts an alpha-helical but dynamically disordered conformation; however, the three-dimensional conformation is stabilized when tBID engages its BH3 domain in the BH3-binding hydrophobic groove of BCL-XL to form a stable heterodimeric complex. Characterization of the binding thermodynamics by ITC reveals that the interaction between tBID and BCL-XL is driven by enthalpy but disfavored by the entropy associated with the conformational order induced in tBID upon binding BCL-XL.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.