ArticleMolecular & cellular biomechanics : MCB2009
Hypertrophic gene expression induced by chronic stretch of excised mouse heart muscle.
Article in Molecular & cellular biomechanics : MCB, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- RNA-sequencing reveals altered skeletal muscle contraction, E3 ligases, autophagy, apoptosis, and chaperone expression in patients with critical illness myopathy.Skeletal muscle · 2019Article
- Macro- and micromechanical remodelling in the fish atrium is associated with regulation of collagen 1 alpha 3 chain expression.Pflugers Archiv : European journal of physiology · 2018Article
- Plasma N-Terminal Probrain Natriuretic Peptide, Vascular Endothelial Growth Factor, and Cardiac Troponin I as Novel Biomarkers of Hypertensive Disease and Target Organ Damage in Cats.Journal of veterinary internal medicine · 2017Article
- Model of Human Fetal Growth in Hypoplastic Left Heart Syndrome: Reduced Ventricular Growth Due to Decreased Ventricular Filling and Altered Shape.Frontiers in pediatrics · 2017Article
- Mechano-signalling pathways in an experimental intensive critical illness myopathy model.The Journal of physiology · 2016Article
- Timing and magnitude of systolic stretch affect myofilament activation and mechanical work.American journal of physiology. Heart and circulatory physiology · 2014Article
- Novel role for vinculin in ventricular myocyte mechanics and dysfunction.Biophysical journal · 2013Article
- Sparing of muscle mass and function by passive loading in an experimental intensive care unit model.The Journal of physiology · 2013Article
- Excess protein O-GlcNAcylation and the progression of diabetic cardiomyopathy.American journal of physiology. Regulatory, integrative and comparative physiology · 2012Article
- Deletion of Drosophila muscle LIM protein decreases flight muscle stiffness and power generation.American journal of physiology. Cell physiology · 2011Article
- Hypertrophy, gene expression, and beating of neonatal cardiac myocytes are affected by microdomain heterogeneity in 3D.Biomedical microdevices · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Altered mechanical stress and strain in cardiac myocytes induce modifications in gene expression that affects cardiac remodeling and myocyte contractile function. To study the mechanisms of mechanotransduction in cardiomyocytes, probing alterations in mechanics and gene expression has been an effective strategy. However, previous studies are self-limited due to the general use of isolated neonatal rodent myocytes or intact animals. The main goal of this study was to develop a novel tissue culture chamber system for mouse myocardium that facilitates loading of cardiac tissue, while measuring tissue stress and deformation within a physiological environment. Intact mouse right ventricular papillary muscles were cultured in controlled conditions with superfusate at 95% O2/ 5% CO2, and 34 degrees C, such that cell to extracellular matrix adhesions as well as cell to cell adhesions were undisturbed and both passive and active mechanical properties were maintained without significant changes. The system was able to measure the induction of hypertrophic markers (BNP, ANP) in tissue after 2 hrs and 5 hrs of stretch. ANP induction was highly correlated with the diastolic load of the muscle but not with developed systolic load. Load induced ANP expression was blunted in muscles from muscle-LIM protein knockout mice, in which defective mechanotransduction pathways have been predicted.
Indexed as
Identifiers
19670825PMC3272304W41179528What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.