ArticlePlant molecular biology2009
Recruitment of AtWHY1 and AtWHY3 by a distal element upstream of the kinesin gene AtKP1 to mediate transcriptional repression.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants.Annals of botany · 2024Review
- WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition.The Biochemical journal · 2023Article
- WHIRLY protein functions in plants.Food and energy security · 2023Review
- Genome-wide identification, phylogenetic, and expression analysis under abiotic stress conditions of Whirly (WHY) gene family in Medicago sativa L.Scientific reports · 2022Article
- WHIRLY1 functions in the nucleus to regulate barley leaf development and associated metabolite profiles.The Biochemical journal · 2022Article
- WHIRLIES Are Multifunctional DNA-Binding Proteins With Impact on Plant Development and Stress Resistance.Frontiers in plant science · 2022Review
- Dual-Localized WHIRLY1 Affects Salicylic Acid Biosynthesis via Coordination of ISOCHORISMATE SYNTHASE1, PHENYLALANINE AMMONIA LYASE1, andPlant physiology · 2020Article
- Triple-localized WHIRLY2 Influences Leaf Senescence and Silique Development via Carbon Allocation.Plant physiology · 2020Article
- Organellar protein multi-functionality and phenotypic plasticity in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Comparative Proteomic Analysis of Coregulation of CIPK14 and WHIRLY1/3 Mediated Pale Yellowing of Leaves inInternational journal of molecular sciences · 2018Article
- WHIRLY1 Occupancy Affects Histone Lysine Modification andFrontiers in plant science · 2018Article
- Mechanism of Dual Targeting of the Phytochrome Signaling Component HEMERA/pTAC12 to Plastids and the Nucleus.Plant physiology · 2017Article
- Nuclear-encoded factors associated with the chloroplast transcription machinery of higher plants.Frontiers in plant science · 2014Review
- Dynamic composition, shaping and organization of plastid nucleoids.Frontiers in plant science · 2014Review
- The single-stranded DNA-binding protein WHIRLY1 represses WRKY53 expression and delays leaf senescence in a developmental stage-dependent manner in Arabidopsis.Plant physiology · 2013Article
- The requirement of WHIRLY1 for embryogenesis is dependent on genetic background in maize.PloS one · 2013Article
- A conserved lysine residue of plant Whirly proteins is necessary for higher order protein assembly and protection against DNA damage.Nucleic acids research · 2012Article
- Plastid Located WHIRLY1 Enhances the Responsiveness of Arabidopsis Seedlings Toward Abscisic Acid.Frontiers in plant science · 2012Article
- Dual targeting and retrograde translocation: regulators of plant nuclear gene expression can be sequestered by plastids.International journal of molecular sciences · 2012Review
- Transcript profiling of crown rootless1 mutant stem base reveals new elements associated with crown root development in rice.BMC genomics · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 43-bp distal element, the AtKP1-related element (KPRE), was previously shown to repress the promoter activity of the kinesin gene AtKP1 in Arabidopsis thaliana. In order to identify KPRE-binding factor 1 (KBF1), a combination of ion-exchange chromatography, gel-filtration chromatography and DNA-affinity chromatography was used to purify KBF1 from whole cell extracts of Arabidopsis seedlings. Mass spectrometric identification showed that KBF1 contains two members of the whirly family of transcription factors, AtWHY1 and AtWHY3. KBF1 is a single and double-stranded DNA-binding factor. A ChIP assay showed that AtWHY1 and AtWHY3 bind to the upstream region of AtKP1 gene in vivo. Over-expression of AtWHY1 and AtWHY3 led to an obvious decrease of AtKP1 transcripts, based on quantitative real-time PCR analysis. Interestingly, salicylic acid treatment resulted in an increase of AtWHY1 and AtWHY3 transcripts, and a decrease of AtKP1 transcripts. Thus, AtWHY1 and AtWHY3, as two components of KBF1, can be recruited at the KPRE site to mediate the transcriptional repression of AtKP1. Our results prove that AtKP1 is a new downstream target of the whirly family of transcription factors.
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