ArticleClinical ophthalmology (Auckland, N.Z.)2007
Effect of proinflammatory cytokines on the human MUC5AC promoter activity in vitro and in vivo.
Article in Clinical ophthalmology (Auckland, N.Z.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- Eucalyptol-Limonene-Pinene Attenuates Mucus Hypersecretion and Inflammation via Multitarget Mechanisms in Chronic Rhinosinusitis With Nasal Polyps.Allergy, asthma & immunology research · 2026Article
- Cytokine Storm and Mucus Hypersecretion in COVID-19: Review of Mechanisms.Journal of inflammation research · 2021Review
- Toxicological effects of urban particulate matter on corneal and conjunctival epithelial cells.Toxicological research · 2020Article
- Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery.PloS one · 2020Article
- Lyn mitigates mouse airway remodeling by downregulating the TGF-β3 isoform in house dust mite models.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Lipopolysaccharides induces MUC5AC overproduction in human nasal epithelium.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2013Article
- Notch signaling prevents mucous metaplasia in mouse conducting airways during postnatal development.Development (Cambridge, England) · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the effect of inflammatory cytokines on the activity of the human MUC5AC promoter in vitro and in vivo.
methodsConjunctival epithelial cells transfected with MUC5AC-luciferase plasmids challenged with different cytokines (tumor necrosis factor-alpha [TNF-alpha], interleukin [IL]-1beta, IL-2, IL-6, and IL-8) at various concentration (1, 10, 20, 50, 100, 200, 500, 1000, and 2000 pg/mL) for 24 hours. A Helio Gene Gun system (Bio-Rad Laboratories, Hercules, CA, USA) was used to deliver MUC5AC-luciferase plasmids into rabbit conjunctivas, which were also challenged with these cytokines (1000 pg/mL) at the frequency of every six hours for 48 hours. The activity of MUC5AC-luciferase was then evaluated using the luciferase assay.
resultsResults of the studies demonstrated that IL-1beta and TNF-alpha upregulated the activity of MUC5AC-luciferase in cultured conjunctival cells, while IL-2, IL-6, and IL-8 had no effect. In rabbit conjunctival tissues, TNF-alpha, IL-1beta, IL-2, IL-8, and IL-6 significantly upregulated MUC5AC gene expression.
conclusionsThis suggests that MUC5AC mucin gene expression is regulated by proinflammatory cytokines, which could have implications in ocular surface disorders.
Indexed as
Identifiers
19668469PMC2699983W33175703What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.