ArticleClinical ophthalmology (Auckland, N.Z.)2008
A comparison of pneumolysin activity and concentration in vitro and in vivo in a rabbit endophthalmitis model.
Article in Clinical ophthalmology (Auckland, N.Z.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 1 citations in OpenAlex.
- Hemolytic Activity and Quantitative Characteristic of Pneumolysin Depending on the Culture Medium of Streptococcus pneumoniae Strains.Bulletin of experimental biology and medicine · 2026Article
- Pneumococcal pneumonia is driven by increased bacterial turnover due to bacteriocin-mediated intra-strain competition.Communications biology · 2024Article
- Glucose and Oxygen Levels Modulate the Pore-Forming Effects of Cholesterol-Dependent Cytolysin Pneumolysin fromToxins · 2024Article
- A Pyrrhic Victory: The PMN Response to Ocular Bacterial Infections.Microorganisms · 2019Review
- A Transcriptional Activator of Ascorbic Acid Transport inMicroorganisms · 2019Article
- Article
- Culture-Proven Endophthalmitis After Intravitreal Injection: A 10-Year Analysis.Ophthalmic surgery, lasers & imaging retina · 2019Article
- Necroptotic Cell Death Promotes Adaptive Immunity Against Colonizing Pneumococci.Frontiers in immunology · 2019Article
- The Role of Pneumococcal Virulence Factors in Ocular Infectious Diseases.Interdisciplinary perspectives on infectious diseases · 2018Review
- Observational
- Activation-dependent modulation of Streptococcus pneumoniae-mediated death in human lymphocytes.Pathogens and disease · 2017Article
- Modeling intraocular bacterial infections.Progress in retinal and eye research · 2016Review
- Differential bacterial gene expression during experimental pneumococcal endophthalmitis.Ophthalmic research · 2015Article
- Role of TLR5 and flagella in bacillus intraocular infection.PloS one · 2014Article
- 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- Moxifloxacin and cholesterol combined treatment of pneumococcal keratitis.Current eye research · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study was to determine whether the in vitro activity and concentration of Streptococcus pneumoniae pneumolysin correlated to the pathogenesis of S. pneumoniae endophthalmitis. Five S. pneumoniae clinical endophthalmitis strains were grown in media to similar optical densities (OD), and extracellular milieu was tested for pneumolysin activity by hemolysis of rabbit red blood cells. Pneumolysin concentration was determined using a sandwich ELISA. Rabbit vitreous was injected with 10(2) colony-forming units (CFU) of 1 of 2 different strains with low hemolytic activity (n = 10 and 12 for strains 4 and 5, respectively) or 1 of 3 different strains with high hemolytic activity (n = 12 per strain). Pathogenesis of endophthalmitis infection was graded by slit lamp examination (SLE) at 24 hours post-infection. Bacteria were recovered from infected vitreous and quantitated. The SLE scores of eyes infected with strains having high hemolytic activity were significantly higher than the scores of those infected with strains having low hemolytic activity (P < 0.05). Pneumolysin concentration in vitro, however, did not correlate with hemolysis or severity of endophthalmitis. Bacterial concentrations from the vitreous infected with 4 of the strains were not significantly different (P > 0.05). These data suggest that pneumolysin hemolytic activity in vitro directly correlates to the pathogenesis of S. pneumoniae endophthalmitis. The protein concentration of pneumolysin, however, is not a reliable indicator of pneumolysin activity.
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