ArticlePloS one2009
Testing and validation of high density resequencing microarray for broad range biothreat agents detection.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Sensitivity and specificity of diagnostic tests for Lassa fever: a systematic review.BMC infectious diseases · 2019Pooled it
- A narrative review of wastewater surveillance: pathogens of concern, applications, detection methods, and challenges.Frontiers in public health · 2024Review
- Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray.Frontiers in microbiology · 2021Article
- Baseline mapping of Lassa fever virology, epidemiology and vaccine research and development.NPJ vaccines · 2018Review
- DNA Microarray Platform for Detection and Surveillance of Viruses Transmitted by Small Mammals and Arthropods.PLoS neglected tropical diseases · 2016Article
- Viral Hemorrhagic Fever Diagnostics.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Review
- Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water.Biomicrofluidics · 2016Article
- Waterborne pathogens: detection methods and challenges.Pathogens (Basel, Switzerland) · 2015Review
- Sequence variability and geographic distribution of Lassa virus, Sierra Leone.Emerging infectious diseases · 2015Article
- Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens.Frontiers in microbiology · 2015Article
- Molecular diagnostic and genetic characterization of highly pathogenic viruses: application during Crimean-Congo haemorrhagic fever virus outbreaks in Eastern Europe and the Middle East.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2013Article
- Article
- Multipurpose instantaneous microarray detection of acute encephalitis causing viruses and their expression profiles.Current microbiology · 2012Review
- Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting.Health research policy and systems · 2012Article
- Development of real-time PCR array for simultaneous detection of eight human blood-borne viral pathogens.PloS one · 2012Article
- High-throughput retina-array for screening 93 genes involved in inherited retinal dystrophy.Investigative ophthalmology & visual science · 2011Article
- Microarrays for pathogen detection and analysis.Briefings in functional genomics · 2011Review
- Isolation of a highly thermal stable lama single domain antibody specific for Staphylococcus aureus enterotoxin B.BMC biotechnology · 2011Article
- Application of a broad-range resequencing array for detection of pathogens in desert dust samples from Kuwait and Iraq.Applied and environmental microbiology · 2011Article
- Genome-wide profiling of microRNAs in adipose mesenchymal stem cell differentiation and mouse models of obesity.PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and effective detection and identification of emerging microbiological threats and potential biowarfare agents is very challenging when using traditional culture-based methods. Contemporary molecular techniques, relying upon reverse transcription and/or polymerase chain reaction (RT-PCR/PCR) provide a rapid and effective alternative, however, such assays are generally designed and optimized to detect only a limited number of targets, and seldom are capable of differentiation among variants of detected targets. To meet these challenges, we have designed a broad-range resequencing pathogen microarray (RPM) for detection of tropical and emerging infectious agents (TEI) including biothreat agents: RPM-TEI v 1.0 (RPM-TEI). The scope of the RPM-TEI assay enables detection and differential identification of 84 types of pathogens and 13 toxin genes, including most of the class A, B and C select agents as defined by the Centers for Disease Control and Prevention (CDC, Atlanta, GA). Due to the high risks associated with handling these particular target pathogens, the sensitivity validation of the RPM-TEI has been performed using an innovative approach, in which synthetic DNA fragments are used as templates for testing the assay's limit of detection (LOD). Assay specificity and sensitivity was subsequently confirmed by testing with full-length genomic nucleic acids of selected agents. The LOD for a majority of the agents detected by RPM-TEI was determined to be at least 10(4) copies per test. Our results also show that the RPM-TEI assay not only detects and identifies agents, but is also able to differentiate near neighbors of the same agent types, such as closely related strains of filoviruses of the Ebola Zaire group, or the Machupo and Lassa arenaviruses. Furthermore, each RPM-TEI assay results in specimen-specific agent gene sequence information that can be used to assess pathogenicity, mutations, and virulence markers, results that are not generally available from multiplexed RT-PCR/PCR-based detection assays.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.