Evidence map›Paper›PMID 19668224›Full record

ReviewNature reviews. Microbiology2009

Cryo-electron tomography of bacteria: progress, challenges and future prospects.

Jacqueline L S Milne, Sriram Subramaniam

Abstract readReview
In one paragraph

Review in Nature reviews. Microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Neurons as a model system for cryo-electron tomography.Journal of structural biology: X · 2022
    Article
  5. Evolution of the analytical scattering model of liveJournal of applied crystallography · 2021
    Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. RNA Localization in Bacteria.Microbiology spectrum · 2018
    Review
  12. IgG Antibody 3D Structures and Dynamics.Antibodies (Basel, Switzerland) · 2018
    Review
  13. IUCrJ · 2017
    Article
  14. Bacterial Cell Mechanics.Biochemistry · 2017
    Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Minicells, Back in Fashion.Journal of bacteriology · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Jacqueline L S MilneLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Sriram Subramaniam

Funding

Technology Development for 3D Electron MicroscopyZIABC010826 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SUBRAMANIAM, SRIRAM · 2009 to 2019
$9.1M
Imaging cellular assemblies with three-dimensional electron microscopyZIABC010278 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SUBRAMANIAM, SRIRAM · 2009 to 2019
$8.0M
Molecular structures of membrane protein assembliesZIABC010824 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SUBRAMANIAM, SRIRAM · 2009 to 2019
$8.0M
Structural Studies of Metabolic Enzymes and Their RegulationZIABC010827 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI MILNE, JACQUELINE · 2009 to 2019
$2.7M
Structural Analysis of Macromolecular Complexes by Electron MicroscopyZIABC010412 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI MILNE, JACQUELINE · 2009 to 2019
$2.7M
Imaging cellular assemblies with three-dimensional EMZ01BC010278 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SUBRAMANIAM, SRIRAM · 1999 to 2008
$1.1M
Technology Development for 3D Electron MicroscopyZ01BC010826 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SUBRAMANIAM, SRIRAM · 2007 to 2008
$1.1M
Molecular structure of the bacterial chemotaxis apparatusZ01BC010824 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SUBRAMANIAM, SRIRAM · 2007 to 2008
$1.1M
Structural Studies of Bacterial Architecture Using Electron TomographyZ01BC010827 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI MILNE, JACQUELINE · 2007 to 2008
$575k
Intramural NIH HHS Z01 BC010278Intramural NIH HHS Z01 BC010826Intramural NIH HHS ZIA BC010824
6 · The paper itself

Abstract

Recent advances in three-dimensional electron microscopy provide remarkable tools to image the interior of bacterial cells. Glimpses of cells at resolutions that are 1-2 orders of magnitude higher than those currently attained with light microscopy can now be obtained with cryo-electron tomography, especially when used in combination with new tools for image averaging. This Review highlights recent advances in this area and provides an assessment of the general applicability, current limitations and type of structural information that can be obtained about the organization of intact cells using tomography. Possible future directions for whole cell imaging are also discussed.

Indexed as

BacteriaCryoelectron MicroscopyElectron Microscope TomographyImaging, Three-Dimensional

Identifiers

PMID19668224
PMCPMC6993139

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.