ArticleProceedings of the National Academy of Sciences of the United States of America2009
Phosphatidylinositol 4,5-bisphosphate (PIP2) stimulates the electrogenic Na/HCO3 cotransporter NBCe1-A expressed in Xenopus oocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- The SLC-ome of membrane transport: from molecular discovery to physiology and clinical applications.Physiological reviews · 2026Review
- Transport mechanism of the SLC4 proteins-Lessons from recent structural and computational studies.The Journal of biological chemistry · 2026Review
- Molecular dynamics simulations of lipid-protein interactions in SLC4 proteins.Biophysical journal · 2024Article
- Structural and functional insights into the lipid regulation of human anion exchanger 2.Nature communications · 2024Article
- Structural insights into the conformational changes of BTR1/SLC4A11 in complex with PIPNature communications · 2023Article
- Endoplasmic Reticulum-Plasma Membrane Contact Sites as an Organizing Principle for Compartmentalized Calcium and cAMP Signaling.International journal of molecular sciences · 2021Review
- The Fundamental Role of Bicarbonate Transporters and Associated Carbonic Anhydrase Enzymes in Maintaining Ion and pH Homeostasis in Non-Secretory Organs.International journal of molecular sciences · 2020Review
- Distinct functional properties of two electrogenic isoforms of the SLC34 Na-Pi cotransporter.Physiological reports · 2019Article
- Emerging Diversity in Lipid-Protein Interactions.Chemical reviews · 2019Review
- Lipids at membrane contact sites: cell signaling and ion transport.EMBO reports · 2017Review
- Phosphatidylinositol 4,5-bisphosphate degradation inhibits the Na+/bicarbonate cotransporter NBCe1-B and -C variants expressed in Xenopus oocytes.The Journal of physiology · 2015Article
- Regulators of Slc4 bicarbonate transporter activity.Frontiers in physiology · 2015Review
- Molecular mechanisms and regulation of urinary acidification.Comprehensive Physiology · 2014Review
- Cation-coupled bicarbonate transporters.Comprehensive Physiology · 2014Review
- NBCe1 as a model carrier for understanding the structure-function properties of Na⁺ -coupled SLC4 transporters in health and disease.Pflugers Archiv : European journal of physiology · 2014Review
- Proximal renal tubular acidosis mediated by mutations in NBCe1-A: unraveling the transporter's structure-functional properties.Frontiers in physiology · 2013Review
- Comparison between drug-induced and K⁺-induced changes in molar acid extrusion fluxes (JH⁺) and in energy consumption rates in astrocytes.Neurochemical research · 2013Article
- Structure, function, and regulation of the SLC4 NBCe1 transporter and its role in causing proximal renal tubular acidosis.Current opinion in nephrology and hypertension · 2013Review
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- The divergence, actions, roles, and relatives of sodium-coupled bicarbonate transporters.Physiological reviews · 2013Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Bicarbonate transporters are regulated by signaling molecules/ions such as protein kinases, ATP, and Ca(2+). While phospholipids such as PIP(2) can stimulate Na-H exchanger activity, little is known about phospholipid regulation of bicarbonate transporters. We used the patch-clamp technique to study the function and regulation of heterologously expressed rat NBCe1-A in excised macropatches from Xenopus laevis oocytes. Exposing the cytosolic side of inside-out macropatches to a 5% CO(2)/33 mM HCO(3)(-) solution elicited a mean inward current of 14 pA in 74% of macropatches attached to pipettes (-V(p) = -60 mV) containing a low-Na(+), nominally HCO(3)(-)-free solution. The current was 80-90% smaller in the absence of Na(+), approximately 75% smaller in the presence of 200 microM DIDS, and absent in macropatches from H(2)O-injected oocytes. NBCe1-A currents exhibited time-dependent rundown that was inhibited by removing Mg(2+) in the presence or absence of vanadate and F(-) to reduce general phosphatase activity. Applying 5 or 10 microM PIP(2) (diC8) in the presence of HCO(3)(-) induced an inward current in 54% of macropatches from NBC-expressing, but not H(2)O-injected oocytes. PIP(2)-induced currents were HCO(3)(-)-dependent and somewhat larger following more NBCe1-A rundown, 62% smaller in the absence of Na(+), and 90% smaller in the presence of 200 microM DIDS. The polycation neomycin (250-500 microM) reduced the PIP(2)-induced inward current by 69%; spermine (100 microM) reduced the current by 97%. Spermine, poly-D-lysine, and neomycin all reduced the baseline HCO(3)(-)-induced inward currents by as much as 85%. In summary, PIP(2) stimulates NBCe1-A activity, and phosphoinositides are regulators of bicarbonate transporters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.