ArticleInfection and immunity2009
Tricarboxylic acid cycle-dependent attenuation of Staphylococcus aureus in vivo virulence by selective inhibition of amino acid transport.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 75 citations in OpenAlex.
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- Immune dysfunction during S. aureus biofilm-associated implant infections: opportunities for novel therapeutic strategies.NPJ biofilms and microbiomes · 2025Review
- Proteomic signatures ofBiofilm · 2025Article
- Proteomic and metabolomic profiling of methicillin resistant versus methicillin sensitiveFrontiers in microbiology · 2024Article
- Comparative Analysis of Transcriptome and Proteome Revealed the Common Metabolic Pathways Induced by Prevalent ESBL Plasmids inInternational journal of molecular sciences · 2023Article
- Impact of the pentose phosphate pathway on metabolism and pathogenesis of Staphylococcus aureus.PLoS pathogens · 2023Article
- Metabolomic profiling of bacterial biofilm: trends, challenges, and an emerging antibiofilm target.World journal of microbiology & biotechnology · 2023Review
- Article
- GltS regulates biofilm formation in methicillin-resistant Staphylococcus aureus.Communications biology · 2022Article
- RNase III CLASH in MRSA uncovers sRNA regulatory networks coupling metabolism to toxin expression.Nature communications · 2022Article
- Linking Multi-Omics to Wheat Resistance Types toInternational journal of molecular sciences · 2022Review
- Proteomic Profiles of Staphylococcus aureus Strains Associated with Subclinical Bovine Mastitis.Current microbiology · 2022Article
- Relationship between L-lactate dehydrogenase and multidrug resistance in Staphylococcus xylosus.Archives of microbiology · 2021Article
- TheMicrobiology spectrum · 2021Article
- Plasmid-Chromosome Crosstalk inFrontiers in cellular and infection microbiology · 2021Article
- Exploring amino acid and peptide transporters as therapeutic targets to attenuate virulence and antibiotic resistance in Staphylococcus aureus.PLoS pathogens · 2021Article
- Identification of the main glutamine and glutamate transporters in Staphylococcus aureus and their impact on c-di-AMP production.Molecular microbiology · 2020Article
- Role of respiratory NADH oxidation in the regulation of Staphylococcus aureus virulence.EMBO reports · 2020Article
- Microbial Metabolic Genes Crucial forFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Staphylococci are the leading causes of endovascular infections worldwide. Commonly, these infections involve the formation of biofilms on the surface of biomaterials. Biofilms are a complex aggregation of bacteria commonly encapsulated by an adhesive exopolysaccharide matrix. In staphylococci, this exopolysaccharide matrix is composed of polysaccharide intercellular adhesin (PIA). PIA is synthesized when the tricarboxylic acid (TCA) cycle is repressed. The inverse correlation between PIA synthesis and TCA cycle activity led us to hypothesize that increasing TCA cycle activity would decrease PIA synthesis and biofilm formation and reduce virulence in a rabbit catheter-induced model of biofilm infection. TCA cycle activity can be induced by preventing staphylococci from exogenously acquiring a TCA cycle-derived amino acid necessary for growth. To determine if TCA cycle induction would decrease PIA synthesis in Staphylococcus aureus, the glutamine permease gene (glnP) was inactivated and TCA cycle activity, PIA accumulation, biofilm forming ability, and virulence in an experimental catheter-induced endovascular biofilm (endocarditis) model were determined. Inactivation of this major glutamine transporter increased TCA cycle activity, transiently decreased PIA synthesis, and significantly reduced in vivo virulence in the endocarditis model in terms of achievable bacterial densities in biofilm-associated cardiac vegetations, kidneys, and spleen. These data confirm the close linkage of TCA cycle activity and virulence factor production and establish that this metabolic linkage can be manipulated to alter infectious outcomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.