ArticleDevelopment (Cambridge, England)2009
hnRNP I is required to generate the Ca2+ signal that causes egg activation in zebrafish.
Article in Development (Cambridge, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Maternal Huluwa regulates postfertilization microtubule array organization for asymmetrical transport of dorsal determinants in zebrafish.Nature communications · 2026Article
- Protease-mediated activation of Par2 elicits calcium waves during zebrafish egg activation and blastomere cleavage.PLoS biology · 2025Article
- Membrane progesterone receptor e (paqr9) is necessary for chorion elevation in zebrafish.Fish physiology and biochemistry · 2025Article
- Maternal provisioning and zygotic activation: transcriptomic dynamics of early atlantic halibut (Frontiers in cell and developmental biology · 2025Article
- Phenogenetics of cortical granule dynamics during zebrafish oocyte-to-embryo transition.Frontiers in cell and developmental biology · 2025Review
- Maternal regulation of the vertebrate oocyte-to-embryo transition.PLoS genetics · 2024Article
- Novel PLCZ1 mutation caused polyspermy during in vitro fertilization.Asian journal of andrology · 2024Article
- Single-Cell RNA Sequencing (scRNA-seq) Identifies L1CAM as a Key Mediator between Epithelial Tuft Cell and Innate Lymphoid Cell in the Colon ofBiomedicines · 2023Article
- PTBP1 controls intestinal epithelial regeneration through post-transcriptional regulation of gene expression.Nucleic acids research · 2023Article
- Knockin' on Egg's Door: Maternal Control of Egg Activation That Influences Cortical Granule Exocytosis in Animal Species.Frontiers in cell and developmental biology · 2021Review
- The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish.Scientific reports · 2020Article
- The Zebrafish Perivitelline Fluid Provides Maternally-Inherited Defensive Immunity.Biomolecules · 2020Article
- Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish.Communications biology · 2020Article
- Maternal Larp6 controls oocyte development, chorion formation and elevation.Development (Cambridge, England) · 2020Article
- Fishing forward and reverse: Advances in zebrafish phenomics.Mechanisms of development · 2018Review
- Double maternal-effect: duplicated nucleoplasmin 2 genes, npm2a and npm2b, with essential but distinct functions are shared by fish and tetrapods.BMC evolutionary biology · 2018Article
- hnRNP I regulates neonatal immune adaptation and prevents colitis and colorectal cancer.PLoS genetics · 2017Article
- aura (mid1ip1l) regulates the cytoskeleton at the zebrafish egg-to-embryo transition.Development (Cambridge, England) · 2016Article
- Deep Brain Photoreceptor (val-opsin) Gene Knockout Using CRISPR/Cas Affects Chorion Formation and Embryonic Hatching in the Zebrafish.PloS one · 2016Article
- Kinesin-1 interacts with Bucky ball to form germ cells and is required to pattern the zebrafish body axis.Development (Cambridge, England) · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Egg activation is an important cellular event required to prevent polyspermy and initiate development of the zygote. Egg activation in all animals examined is elicited by a rise in free Ca(2+) in the egg cytosol at fertilization. This Ca(2+) rise is crucial for all subsequent egg activation steps, such as cortical granule exocytosis, which modifies the vitelline membrane to prevent polyspermy. The cytosolic Ca(2+) rise is primarily initiated by inositol 1,4,5-trisphosphate (IP(3))-mediated Ca(2+) release from the endoplasmic reticulum. The genes involved in regulating the IP(3)-mediated Ca(2+) release during egg activation remain largely unknown. Here we report on a zebrafish maternal-effect mutant, brom bones, which is defective in the cytosolic Ca(2+) rise and subsequent egg activation events, including cortical granule exocytosis and cytoplasmic segregation. We show that the egg activation defects in brom bones can be rescued by providing Ca(2+) or the Ca(2+)-release messenger IP(3), suggesting that brom bones is a regulator of IP(3)-mediated Ca(2+) release at fertilization. Interestingly, brom bones mutant embryos also display defects in dorsoventral axis formation accompanied by a disorganized cortical microtubule network, which is known to be crucial for dorsal axis formation. We provide evidence that the impaired microtubule organization is associated with non-exocytosed cortical granules from the earlier egg activation defect. Positional cloning of the brom bones gene reveals that a premature stop codon in the gene encoding hnRNP I (referred to here as hnrnp I) underlies the abnormalities. Our studies therefore reveal an important new role of hnrnp I in regulating the fundamental process of IP(3)-mediated Ca(2+) release at egg activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.