ArticleEukaryotic cell2009
A single dicer gene is required for efficient gene silencing associated with two classes of small antisense RNAs in Mucor circinelloides.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 79 citations in OpenAlex.
- Clade-wide fungal proteome analysis reveals structure-function conservation in divergent Dicer proteins.Computational and structural biotechnology journal · 2025Article
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- Review
- Heterochromatin and RNAi act independently to ensure genome stability in Mucorales human fungal pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Similar Characteristics of siRNAs of Plant Viruses Which Replicate in Plant and Fungal Hosts.Biology · 2022Article
- Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis.International journal of molecular sciences · 2022Review
- Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Role of Dicer-Dependent RNA Interference in Regulating Mycoparasitic Interactions.Microbiology spectrum · 2021Article
- A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakeslea trispora.Biotechnology letters · 2021Article
- The RNAi Mechanism Regulates a New Exonuclease Gene Involved in the Virulence of Mucorales.International journal of molecular sciences · 2021Article
- A Mucoralean White Collar-1 Photoreceptor Controls Virulence by Regulating an Intricate Gene Network during Host Interactions.Microorganisms · 2021Article
- A Novel DCL2-Dependent Micro-Like RNAFrontiers in microbiology · 2021Article
- The Evolutionary Significance of RNAi in the Fungal Kingdom.International journal of molecular sciences · 2020Review
- Review
- Epigenetic mechanisms of drug resistance in fungi.Fungal genetics and biology : FG & B · 2019Review
- Extracellular Vesicle-Mediated RNA Release inmSphere · 2019Article
- Genetic tools for investigating Mucorales fungal pathogenesis.Current clinical microbiology reports · 2018Article
- Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation.Current protocols in microbiology · 2018Article
- Dicer-Like Proteins Regulate Sexual Development via the Biogenesis of Perithecium-Specific MicroRNAs in a Plant Pathogenic FungusFrontiers in microbiology · 2018Article
- Construction of a Recyclable Genetic Marker and Serial Gene Deletions in the Human Pathogenic MucoralesG3 (Bethesda, Md.) · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA silencing in the zygomycete Mucor circinelloides exhibits uncommon features, such as induction by self-replicative sense transgenes and the accumulation of two size classes of antisense small interfering RNAs (siRNAs). To investigate whether this silencing phenomenon follows the rules of a canonical RNA-silencing mechanism, we used hairpin RNA (hpRNA)-producing constructs as silencing triggers and analyzed the efficiency and stability of silencing in different genetic backgrounds. We show here that the dsRNA-induced silencing mechanism is also associated with the accumulation of two sizes of antisense siRNAs and that this mechanism is not mediated by the previously known dcl-1 (dicer-like) gene, which implies the existence of an additional dicer gene. An M. circinelloides dcl-2 gene was cloned and characterized, and the corresponding null mutant was generated by gene replacement. This mutant is severely impaired in the silencing mechanism induced by self-replicative sense or inverted-repeat transgenes, providing the first genetic evidence of a canonical silencing mechanism in this class of fungus and pointing to a role for dcl-2 in the mechanism. Moreover, a functional dcl-2 gene is required for the normal accumulation of the two sizes of antisense RNAs, as deduced from the analysis of dcl-2(-) transformants containing hpRNA-expressing plasmids. In addition to its critical role in transgene-induced silencing, the dcl-2 gene seems to play a role in the control of vegetative development, since the dcl-2 null mutants showed a significant decrease in their production of asexual spores.
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