ArticleApplied and environmental microbiology2009
Transcriptomic response of Escherichia coli O157:H7 to oxidative stress.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers, 1 of them a synthesis that pooled it.
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Who cites it
77 citing papers in PubMed, 1 synthesis or guideline pooled it, 163 citations in OpenAlex.
- Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR.BMC molecular biology · 2011Pooled it
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- Non-Canonical Aspects of Antibiotics and Antibiotic Resistance.Antibiotics (Basel, Switzerland) · 2024Review
- Antimicrobial Tolerance inAnimals : an open access journal from MDPI · 2024Review
- Use of transcriptomics and genomics to assess the effect of disinfectant exposure on the survival and resistance ofFrontiers in microbiology · 2024Article
- Multicentre genetic diversity study of carbapenem-resistant Enterobacterales: predominance of untypeable pUVA-likeJAC-antimicrobial resistance · 2023Article
- Identification and functional analysis of novel protein-encoding sequences related to stress-resistance.Frontiers in microbiology · 2023Article
- The secondary metabolite hydrogen cyanide protectsFrontiers in microbiology · 2023Article
- Serratia marcescens DUF1471-Containing Protein SrfN Is Needed for Adaptation to Acid and Oxidative Stresses.mSphere · 2022Article
- Stress Response Mechanisms ofFoods (Basel, Switzerland) · 2022Article
- Investigation on the Microbial Diversity of Fresh-Cut Lettuce during Processing and Storage Using High Throughput Sequencing and Their Relationship with Quality.Foods (Basel, Switzerland) · 2022Article
- Metabolic Responses of "Big Six" Escherichia coli in Wheat Flour to Thermal Treatment Revealed by Nuclear Magnetic Resonance Spectroscopy.Applied and environmental microbiology · 2022Article
- Transcriptomic Analysis ofAntioxidants (Basel, Switzerland) · 2022Article
- Deletion of both methionine sulfoxide reductase A and methionine sulfoxide reductase C genes renders Salmonella Typhimurium highly susceptible to hypochlorite stress and poultry macrophages.Molecular biology reports · 2021Article
- Induction of the reactive chlorine-responsive transcription factor RclR in Escherichia coli following ingestion by neutrophils.Pathogens and disease · 2021Article
- The effects of neutrophil-generated hypochlorous acid and other hypohalous acids on host and pathogens.Cellular and molecular life sciences : CMLS · 2021Review
- Uncovering Key Metabolic Determinants of the Drug Interactions Between Trimethoprim and Erythromycin inFrontiers in microbiology · 2021Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlorinated water is commonly used in industrial operations to wash and sanitize fresh-cut, minimally processed produce. Here we compared 42 human outbreak strains that represented nine distinct Escherichia coli O157:H7 genetic lineages (or clades) for their relative resistance to chlorine treatment. A quantitative measurement of resistance was made by comparing the extension of the lag phase during growth of each strain under exposure to sublethal concentrations of sodium hypochlorite in Luria-Bertani or brain heart infusion broth. Strains in clade 8 showed significantly (P < 0.05) higher resistance to chlorine than strains from other clades of E. coli O157:H7. To further explore how E. coli O157:H7 responds to oxidative stress at transcriptional levels, we analyzed the global gene expression profiles of two strains, TW14359 (clade 8; associated with the 2006 spinach outbreak) and Sakai (clade 1; associated with the 1996 radish sprout outbreak), under sodium hypochlorite or hydrogen peroxide treatment. We found over 380 genes were differentially expressed (more than twofold; P < 0.05) after exposure to low levels of chlorine or hydrogen peroxide. Significantly upregulated genes included several regulatory genes responsive to oxidative stress, genes encoding putative oxidoreductases, and genes associated with cysteine biosynthesis, iron-sulfur cluster assembly, and antibiotic resistance. Identification of E. coli O157:H7 strains with enhanced resistance to chlorine decontamination and analysis of their transcriptomic response to oxidative stress may improve our basic understanding of the survival strategy of this human enteric pathogen on fresh produce during minimal processing.
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