ArticleApplied and environmental microbiology2009
Intra- and interspecies conjugal transfer of Tn916-like elements from Lactococcus lactis in vitro and in vivo.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Multiple Independent Origins of TnAntibiotics (Basel, Switzerland) · 2026Article
- Advances in Genetic Transformation of Lactic Acid Bacteria: Overcoming Barriers and Enhancing Plasmid Tools.International journal of molecular sciences · 2025Review
- Genome analysis reveals a biased distribution of virulence and antibiotic resistance genes in the genusApplied and environmental microbiology · 2025Article
- Transfer of the Integrative and Conjugative Element ICEMicrobiology spectrum · 2023Article
- A Novel Mobilizing Tool Based on the Conjugative Transfer System of the IncM Plasmid pCTX-M3.Applied and environmental microbiology · 2020Article
- Dynamics of oxytetracycline and resistance genes in soil under long-term intensive compost fertilization in Northern China.Environmental science and pollution research international · 2019Article
- New Insights on the Role of the pLMST6 Plasmid inFrontiers in microbiology · 2019Article
- Conjugation-Mediated Horizontal Gene Transfer of Clostridium perfringens Plasmids in the Chicken Gastrointestinal Tract Results in the Formation of New Virulent Strains.Applied and environmental microbiology · 2017Article
- Lactic acid bacteria - promising vaccine vectors: possibilities, limitations, doubts.Journal of applied microbiology · 2017Review
- Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain.Applied microbiology and biotechnology · 2017Article
- Draft Genome Sequence of the Plasmid-FreeGenome announcements · 2017Article
- Adhesion of the genome-sequenced Lactococcus lactis subsp. cremoris IBB477 strain is mediated by specific molecular determinants.Applied microbiology and biotechnology · 2016Article
- Rampant Parasexuality Evolves in a Hospital Pathogen during Antibiotic Selection.Molecular biology and evolution · 2015Article
- Characterization of Mobile Staphylococcus equorum Plasmids Isolated from Fermented Seafood That Confer Lincomycin Resistance.PloS one · 2015Article
- Unusual genome complexity in Lactobacillus salivarius JCM1046.BMC genomics · 2014Article
- Update on antibiotic resistance in foodborne Lactobacillus and Lactococcus species.Frontiers in microbiology · 2013Review
- Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.PloS one · 2013Article
- Measuring kinetic dissociation/association constants between Lactococcus lactis bacteria and mucins using living cell probes.Biophysical journal · 2011Article
- Antibiotic resistance determinants in the interplay between food and gut microbiota.Genes & nutrition · 2011Article
- Detection of tetracycline and macrolide resistance determinants in Enterococci of animal and environmental origin using multiplex PCR.Folia microbiologica · 2011Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tetracycline-resistant Lactococcus lactis strains originally isolated from Polish raw milk were analyzed for the ability to transfer their antibiotic resistance genes in vitro, using filter mating experiments, and in vivo, using germfree rats. Four of six analyzed L. lactis isolates were able to transfer tetracycline resistance determinants in vitro to L. lactis Bu2-60, at frequencies ranging from 10(-5) to 10(-7) transconjugants per recipient. Three of these four strains could also transfer resistance in vitro to Enterococcus faecalis JH2-2, whereas no transfer to Bacillus subtilis YBE01, Pseudomonas putida KT2442, Agrobacterium tumefaciens UBAPF2, or Escherichia coli JE2571 was observed. Rats were initially inoculated with the recipient E. faecalis strain JH2-2, and after a week, the L. lactis IBB477 and IBB487 donor strains were introduced. The first transconjugants were detected in fecal samples 3 days after introduction of the donors. A subtherapeutic concentration of tetracycline did not have any significant effect on the number of transconjugants, but transconjugants were observed earlier in animals dosed with this antibiotic. Molecular analysis of in vivo transconjugants containing the tet(M) gene showed that this gene was identical to tet(M) localized on the conjugative transposon Tn916. Primer-specific PCR confirmed that the Tn916 transposon was complete in all analyzed transconjugants and donors. This is the first study showing in vivo transfer of a Tn916-like antibiotic resistance transposon from L. lactis to E. faecalis. These data suggest that in certain cases food lactococci might be involved in the spread of antibiotic resistance genes to other lactic acid bacteria.
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