ArticleApplied and environmental microbiology2009
Inorganic phosphate and sodium ions are cogerminants for spores of Clostridium perfringens type A food poisoning-related isolates.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- The biology and pathogenicity ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- Clostridium septicum manifests a bile salt germinant response mediated by Clostridioides difficile csp gene orthologs.Communications biology · 2024Article
- Identification of Germinants and Expression of Germination Genes inPathogens (Basel, Switzerland) · 2024Article
- Divalent Cation Signaling inMicroorganisms · 2023Article
- Comparison of sporulation and germination conditions forFrontiers in microbiology · 2023Article
- Dormant spores sense amino acids through the B subunits of their germination receptors.Nature communications · 2021Article
- Clostridioides difficile SpoVAD and SpoVAE Interact and Are Required for Dipicolinic Acid Uptake into Spores.Journal of bacteriology · 2021Article
- Sporulation and Germination in Clostridial Pathogens.Microbiology spectrum · 2019Review
- Germinants and Their Receptors in Clostridia.Journal of bacteriology · 2016Review
- Analysis of the Spore Membrane Proteome in Clostridium perfringens Implicates Cyanophycin in Spore Assembly.Journal of bacteriology · 2016Article
- Clostridium perfringens Sporulation and Sporulation-Associated Toxin Production.Microbiology spectrum · 2016Review
- Spore Cortex Hydrolysis Precedes Dipicolinic Acid Release during Clostridium difficile Spore Germination.Journal of bacteriology · 2015Article
- The Clostridium perfringens germinant receptor protein GerKC is located in the spore inner membrane and is crucial for spore germination.Journal of bacteriology · 2013Article
- Bile acid recognition by the Clostridium difficile germinant receptor, CspC, is important for establishing infection.PLoS pathogens · 2013Article
- Transcriptional analysis of temporal gene expression in germinating Clostridium difficile 630 endospores.PloS one · 2013Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium perfringens type A isolates carrying a chromosomal copy of the enterotoxin (cpe) gene are involved in the majority of food poisoning (FP) outbreaks, while type A isolates carrying a plasmid-borne cpe gene are involved in C. perfringens-associated non-food-borne (NFB) gastrointestinal diseases. To cause diseases, C. perfringens spores must germinate and return to active growth. Previously, we showed that only spores of FP isolates were able to germinate with K(+) ions. We now found that the spores of the majority of FP isolates, but none of the NFB isolates, germinated with the cogerminants Na(+) and inorganic phosphate (NaP(i)) at a pH of approximately 6.0. Spores of gerKA-KC and gerAA mutants germinated to a lesser extent and released less dipicolinic acid (DPA) than did wild-type spores with NaP(i). Although gerKB spores germinated to a similar extent as wild-type spores with NaP(i), their rate of germination was lower. Similarly, gerO and gerO gerQ mutant spores germinated slower and released less DPA than did wild-type spores with NaP(i). In contrast, gerQ spores germinated to a slightly lesser extent than wild-type spores but released all of their DPA during NaP(i) germination. In sum, this study identified NaP(i) as a novel nutrient germinant for spores of most FP isolates and provided evidence that proteins encoded by the gerKA-KC operon, gerAA, and gerO are required for NaP(i)-induced spore germination.
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