ArticlePlant physiology2009
Rapid screening for temperature-sensitive alleles in plants.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- A Hot-Swappable Genetic Switch: Building an Inducible and Trackable Functional Assay for the Essential Gene MEDIATOR 21.ACS synthetic biology · 2025Article
- Conditional and tissue-specific approaches to dissect essential mechanisms in plant development.Current opinion in plant biology · 2022Review
- Myosin XI drives polarized growth by vesicle focusing and local enrichment of F-actin in Physcomitrium patens.Plant physiology · 2021Article
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- Conditional genetic screen in Physcomitrella patens reveals a novel microtubule depolymerizing-end-tracking protein.PLoS genetics · 2018Article
- Article
- ATP requirement for chloroplast protein import is set by the Km for ATP hydrolysis of stromal Hsp70 in Physcomitrella patens.The Plant cell · 2014Article
- Comprehensive analysis of differentially expressed rice actin depolymerizing factor gene family and heterologous overexpression of OsADF3 confers Arabidopsis Thaliana drought tolerance.Rice (New York, N.Y.) · 2012Article
- Actin interacting protein1 and actin depolymerizing factor drive rapid actin dynamics in Physcomitrella patens.The Plant cell · 2011Article
- Myosin XI is essential for tip growth in Physcomitrella patens.The Plant cell · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We developed a simple and fast method to identify temperature-sensitive alleles of essential plant genes. We used primary and tertiary structure information to identify residues in the core of the protein of interest. These residues were mutated and tested for temperature sensitivity, taking advantage of the exceptionally rapid 1-week complementation assay in the moss Physcomitrella patens. As test molecules, we selected the actin-binding proteins profilin and actin-depolymerizing factor, because they are essential and their loss-of-function phenotype can be fully rescued. Screening a small number of candidate mutants, we successfully identified temperature-sensitive alleles of both profilin and actin-depolymerizing factor. Plants harboring these alleles grew well at the permissive temperature of 20 degrees C to 25 degrees C but showed a complete loss of function at the restrictive temperature of 32 degrees C. Notably, the profilin mutation identified in the moss gene can be transferred to profilins from other plant species, also rendering them temperature sensitive. The ability to routinely generate temperature-sensitive alleles of essential plant proteins provides a powerful tool for the study of gene function in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.