ArticleProceedings of the National Academy of Sciences of the United States of America2009
Cysteine modification of a specific repressor protein controls the translational status of nucleus-encoded LHCII mRNAs in Chlamydomonas.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 59 citations in OpenAlex.
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- A Light Switch Based on Protein S-Nitrosylation Fine-Tunes Photosynthetic Light Harvesting in Chlamydomonas.Plant physiology · 2016Article
- Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b6f Complex.Plant physiology · 2016Article
- Identification of regulatory network hubs that control lipid metabolism in Chlamydomonas reinhardtii.Journal of experimental botany · 2015Article
- Generation of random mutants to improve light-use efficiency of Nannochloropsis gaditana cultures for biofuel production.Biotechnology for biofuels · 2015Article
- In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity.Frontiers in microbiology · 2015Review
- The link between transcript regulation and de novo protein synthesis in the retrograde high light acclimation response of Arabidopsis thaliana.BMC genomics · 2014Article
- Post-transcriptional control of light-harvesting genes expression under light stress.Plant molecular biology · 2013Article
- Dynamic Light Regulation of Translation Status in Arabidopsis thaliana.Frontiers in plant science · 2012Article
- Cellulose degradation and assimilation by the unicellular phototrophic eukaryote Chlamydomonas reinhardtii.Nature communications · 2012Article
- Construction and evaluation of a whole genome microarray of Chlamydomonas reinhardtii.BMC genomics · 2011Article
- Thiol-based redox switches and gene regulation.Antioxidants & redox signaling · 2011Review
- Time-course global expression profiles of Chlamydomonas reinhardtii during photo-biological H₂ production.PloS one · 2011Article
- Interplay between COPhysiologia plantarumArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytosolic RNA-binding protein NAB1 represses translation of LHCII (light-harvesting complex of photosystem II) encoding mRNAs by sequestration into translationally silent mRNP complexes in the green alga Chlamydomonas reinhardtii. NAB1 contains 2 cysteine residues, Cys-181 and Cys-226, within its C-terminal RRM motif. Modification of these cysteines either by oxidation or by alkylation in vitro was accompanied by a decrease in RNA-binding affinity for the target mRNA sequence. To confirm the relevance of reversible NAB1 cysteine oxidation for the regulation of its activity in vivo, we replaced both cysteines with serines. All examined cysteine single and double mutants exhibited a reduced antenna at PSII caused by a perturbed NAB1 deactivation mechanism, with double mutations and Cys-226 single mutations causing a stronger and more distinctive phenotype compared with the Cys-181 mutation. Our data indicated that the responsible redox control mechanism is mediated by modification of single cysteines. Polysome analyses and RNA co-immunoprecipitation experiments demonstrated the interconnection of the NAB1 thiol state and its activity as a translation repressor in vivo. NAB1 is fully active in its dithiol state and is reversibly deactivated by modification of its cysteines. In summary, this work is an example that cytosolic translation of nucleus encoded photosynthetic genes is regulated via a reversible cysteine-based redox switch in a RNA-binding translation repressor protein.
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