ArticleProceedings of the National Academy of Sciences of the United States of America2009
Structural organization of box C/D RNA-guided RNA methyltransferase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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Who cites it
42 citing papers in PubMed, 65 citations in OpenAlex.
- A sequence-specific RNA acetylation catalyst.Nucleic acids research · 2025Article
- Evolutionary origins of archaeal and eukaryotic RNA-guided RNA modification in bacterial IS110 transposons.Nature microbiology · 2025Article
- A sequence-specific RNA acetylation catalyst.bioRxiv : the preprint server for biology · 2024Article
- Complicated target recognition by archaeal box C/D guide RNAs.Science China. Life sciences · 2024Article
- Advances in the structure and function of the nucleolar protein fibrillarin.Frontiers in cell and developmental biology · 2024Review
- On the origin of the nucleus: a hypothesis.Microbiology and molecular biology reviews : MMBR · 2023Review
- Novel sights on therapeutic, prognostic, and diagnostics aspects of non-coding RNAs in glioblastoma multiforme.Metabolic brain disease · 2023Review
- The roles of NOP56 in cancer and SCA36.Pathology oncology research : POR · 2023Review
- Methylation guide RNAs without box C/D motifs.RNA (New York, N.Y.) · 2022Article
- Article
- Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites.Scientific reports · 2021Article
- High-resolution structure of eukaryotic Fibrillarin interacting with Nop56 amino-terminal domain.RNA (New York, N.Y.) · 2021Article
- Fibrillarin evolution through the Tree of Life: Comparative genomics and microsynteny network analyses provide new insights into the evolutionary history of Fibrillarin.PLoS computational biology · 2020Article
- Functional organization of box C/D RNA-guided RNA methyltransferase.Nucleic acids research · 2020Article
- The multistructural forms of box C/D ribonucleoprotein particles.RNA (New York, N.Y.) · 2018Review
- Sequence determinants of the folding properties of box C/D kink-turns in RNA.RNA (New York, N.Y.) · 2017Article
- Control of box C/D snoRNP assembly by NEMBO reports · 2017Article
- Assembly and trafficking of box C/D and H/ACA snoRNPs.RNA biology · 2017Review
- Box C/D sRNA stem ends act as stabilizing anchors for box C/D di-sRNPs.Nucleic acids research · 2016Article
- Box C/D guide RNAs recognize a maximum of 10 nt of substrates.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Box C/D guide RNAs are abundant noncoding RNAs that primarily function to direct the 2'-O-methylation of specific nucleotides by base-pairing with substrate RNAs. In archaea, a bipartite C/D RNA assembles with L7Ae, Nop5, and the methyltransferase fibrillarin into a modification enzyme with unique substrate specificity. Here, we determined the crystal structure of an archaeal C/D RNA-protein complex (RNP) composed of all 3 core proteins and an engineered half-guide RNA at 4 A resolution, as well as 2 protein substructures at higher resolution. The RNP structure reveals that the C-terminal domains of Nop5 in the dimeric complex provide symmetric anchoring sites for 2 L7Ae-associated kink-turn motifs of the C/D RNA. A prominent protrusion in Nop5 seems to be important for guide RNA organization and function and for discriminating the structurally related U4 snRNA. Multiple conformations of the N-terminal domain of Nop5 and its associated fibrillarin in different structures indicate the inherent flexibility of the catalytic module, suggesting that a swinging motion of the catalytic module is part of the enzyme mechanism. We also built a model of a native C/D RNP with substrate and fibrillarin in an active conformation. Our results provide insight into the overall organization and mechanism of action of C/D RNA-guided RNA methyltransferases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.