ArticleProceedings of the National Academy of Sciences of the United States of America2009
Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Targeting CDK7 increases the stability of Snail to promote the dissemination of colorectal cancer.Cell death and differentiation · 2019Article
- Cdk7: a kinase at the core of transcription and in the crosshairs of cancer drug discovery.Transcription · 2019Review
- The essential and multifunctional TFIIH complex.Protein science : a publication of the Protein Society · 2018Review
- Human TFIIH Kinase CDK7 Regulates Transcription-Associated Chromatin Modifications.Cell reports · 2017Article
- Engineered Covalent Inactivation of TFIIH-Kinase Reveals an Elongation Checkpoint and Results in Widespread mRNA Stabilization.Molecular cell · 2016Article
- THZ1 Reveals Roles for Cdk7 in Co-transcriptional Capping and Pausing.Molecular cell · 2015Article
- Gene looping facilitates TFIIH kinase-mediated termination of transcription.Scientific reports · 2015Article
- The Ssu72 phosphatase mediates the RNA polymerase II initiation-elongation transition.The Journal of biological chemistry · 2014Article
- TFIIH phosphorylation of the Pol II CTD stimulates mediator dissociation from the preinitiation complex and promoter escape.Molecular cell · 2014Article
- The Ess1 prolyl isomerase: traffic cop of the RNA polymerase II transcription cycle.Biochimica et biophysica acta · 2014Review
- Inhibition of post-transcriptional RNA processing by CDK inhibitors and its implication in anti-viral therapy.PloS one · 2014Article
- RNA polymerase II C-terminal domain: Tethering transcription to transcript and template.Chemical reviews · 2013Review
- PNUTS/PP1 regulates RNAPII-mediated gene expression and is necessary for developmental growth.PLoS genetics · 2013Article
- Chemical genetics: budding yeast as a platform for drug discovery and mapping of genetic pathways.Molecules (Basel, Switzerland) · 2012Review
- Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation.The EMBO journal · 2012Article
- Normal and Aberrantly Capped mRNA Decapping.The Enzymes · 2012Article
- Emerging Views on the CTD Code.Genetics research international · 2012Article
- Article
- Requirement of TFIIH kinase subunit Mat1 for RNA Pol II C-terminal domain Ser5 phosphorylation, transcription and mRNA turnover.Nucleic acids research · 2011Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Ser-5 phosphorylation of the RNA polymerase II (Pol II) C-terminal domain by TFIIH kinase has been implicated in critical steps in mRNA synthesis, such as Pol II promoter escape and mRNA 5'-capping. However, the general requirement and precise role of TFIIH kinase in Pol II transcription still remain elusive. Here we use a chemical genetics approach to show that, for a majority of budding-yeast genes, specific inhibition of the yeast TFIIH kinase results in a dramatic reduction in both mRNA level and Ser-5 C-terminal domain phosphorylation. Surprisingly, inhibition of TFIIH kinase activity only partially affected both Pol II density and Ser-2 phosphorylation level. The discrepancy between mRNA level and Pol II density is attributed to the defective 5'-capping, which results in the destabilization of mRNAs. Therefore, contrary to the current belief, our study points strongly toward a minor role of TFIIH kinase in Pol II transcription, and a more significant role in mRNA capping in budding yeast.
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