ArticleThe Journal of biological chemistry2009
Pyriform spidroin 1, a novel member of the silk gene family that anchors dragline silk fibers in attachment discs of the black widow spider, Latrodectus hesperus.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 101 citations in OpenAlex.
- Dual roles of salivary proteins in feeding and silk fiber coating in the spider mite Tetranychus urticae.Communications biology · 2025Article
- Functional Biomaterials Derived from Protein Liquid-Liquid Phase Separation and Liquid-to-Solid Transition.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- A recombinant chimeric spider pyriform-aciniform silk with highly tunable mechanical performance.Materials today. Bio · 2024Article
- Attachment discs of the diving bell spider Argyroneta aquatica.Communications biology · 2023Article
- Protein-Based Biological Materials: Molecular Design and Artificial Production.Chemical reviews · 2023Review
- Review
- The evolutionary history of cribellate orb-weaver capture thread spidroins.BMC ecology and evolution · 2022Article
- Chromosome-level genome assembly of the black widow spider Latrodectus elegans illuminates composition and evolution of venom and silk proteins.GigaScience · 2022Article
- High mechanical property silk produced by transgenic silkworms expressing the spidroins PySp1 and ASG1.Scientific reports · 2021Article
- Hybrid Spider Silk with Inorganic Nanomaterials.Nanomaterials (Basel, Switzerland) · 2020Review
- Expanding Canonical Spider Silk Properties through a DNA Combinatorial Approach.Materials (Basel, Switzerland) · 2020Article
- Spidroins and Silk Fibers of Aquatic Spiders.Scientific reports · 2019Article
- Article
- Imaging and mechanical characterization of different junctions in spider orb webs.Scientific reports · 2019Article
- Silk genes and silk gene expression in the spider Tengella perfuga (Zoropsidae), including a potential cribellar spidroin (CrSp).PloS one · 2018Article
- Article
- From EST to novel spider silk gene identification for production of spidroin-based biomaterials.Scientific reports · 2017Article
- The Nephila clavipes genome highlights the diversity of spider silk genes and their complex expression.Nature genetics · 2017Article
- Sequence basis of Barnacle Cement Nanostructure is Defined by Proteins with Silk Homology.Scientific reports · 2016Article
- Proteomic Evidence for Components of Spider Silk Synthesis from Black Widow Silk Glands and Fibers.Journal of proteome research · 2015Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spiders spin high performance threads that have diverse mechanical properties for specific biological applications. To better understand the molecular mechanism by which spiders anchor their threads to a solid support, we solubilized the attachment discs from black widow spiders and performed in-solution tryptic digests followed by MS/MS analysis to identify novel peptides derived from glue silks. Combining matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry and cDNA library screening, we isolated a novel member of the silk gene family called pysp1 and demonstrate that its protein product is assembled into the attachment disc silks. Alignment of the PySp1 amino acid sequence to other fibroins revealed conservation in the non-repetitive C-terminal region of the silk family. MS/MS analysis also confirmed the presence of MaSp1 and MaSp2, two important components of dragline silks, anchored within the attachment disc materials. Characterization of the ultrastructure of attachment discs using scanning electron microscopy studies support the localization of PySp1 to small diameter fibers embedded in a glue-like cement, which network with large diameter dragline silk threads, producing a strong, adhesive material. Consistent with elevated PySp1 mRNA levels detected in the pyriform gland, MS analysis of the luminal contents extracted from the pyriform gland after tryptic digestion support the assertion that PySp1 represents one of the major constituents manufactured in the pyriform gland. Taken together, our data demonstrate that PySp1 is spun into attachment disc silks to help affix dragline fibers to substrates, a critical function during spider web construction for prey capture and locomotion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.