ArticleFree radical biology & medicine2009
The role of c-Jun phosphorylation in EpRE activation of phase II genes.
Article in Free radical biology & medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Nrf2-mediated anti-inflammatory polarization of macrophages as therapeutic targets for osteoarthritis.Frontiers in immunology · 2022Review
- Role of Oxidative Stress and Nrf2/KEAP1 Signaling in Colorectal Cancer: Mechanisms and Therapeutic Perspectives with Phytochemicals.Antioxidants (Basel, Switzerland) · 2021Review
- Myeloperoxidase Modulates Hydrogen Peroxide Mediated Cellular Damage in Murine Macrophages.Antioxidants (Basel, Switzerland) · 2020Article
- Does Bach1 & c-Myc dependent redox dysregulation of Nrf2 & adaptive homeostasis decrease cancer risk in ageing?Free radical biology & medicine · 2019Review
- Metformin reverses the resistance mechanism of lung adenocarcinoma cells that knocks down the Nrf2 gene.Oncology letters · 2018Article
- Antioxidant response elements: Discovery, classes, regulation and potential applications.Redox biology · 2018Review
- A hypermorphic antioxidant response element is associated with increased MS4A6A expression and Alzheimer's disease.Redox biology · 2018Article
- Sulforaphane reactivates cellular antioxidant defense by inducing Nrf2/ARE/Prdx6 activity during aging and oxidative stress.Scientific reports · 2017Article
- Glutathione S-transferase alpha 4 induction by activator protein 1 in colorectal cancer.Oncogene · 2016Article
- Clopidogrel Protects Endothelium by Hindering TNFα-Induced VCAM-1 Expression through CaMKKβ/AMPK/Nrf2 Pathway.Journal of diabetes research · 2016Article
- Prooxidant and antioxidant properties of salicylaldehyde isonicotinoyl hydrazone iron chelators in HepG2 cells.Biochimica et biophysica acta · 2015Article
- How do nutritional antioxidants really work: nucleophilic tone and para-hormesis versus free radical scavenging in vivo.Free radical biology & medicine · 2014Review
- Cell death and diseases related to oxidative stress: 4-hydroxynonenal (HNE) in the balance.Cell death and differentiation · 2013Review
- Competition of nuclear factor-erythroid 2 factors related transcription factor isoforms, Nrf1 and Nrf2, in antioxidant enzyme induction.Redox biology · 2013Article
- Glutathione synthesis and its role in redox signaling.Seminars in cell & developmental biology · 2012Review
- Platelet-derived growth factor-BB induces cystathionine γ-lyase expression in rat mesangial cells via a redox-dependent mechanism.British journal of pharmacology · 2012Article
- Article
- Reexamination of the electrophile response element sequences and context reveals a lack of consensus in gene function.Biochimica et biophysica acta · 2010Article
- Stress-activated cap'n'collar transcription factors in aging and human disease.Science signaling · 2010Review
- C-Myc is a Nrf2-interacting protein that negatively regulates phase II genes through their electrophile responsive elements.IUBMB life · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The transcription factors that bind to EpRE's play a key role in the regulation of phase II genes. In this study, we examined whether c-Jun, a partner of Nrf2 in binding to EpRE's, requires phosphorylation by JNK for binding and transcriptional activation. We used chromatin immunoprecipitation assays to measure the recruitment of transcription factors to EpRE sequences in NQO2, GCLC, and GCLM; Western analysis for phosphorylation of JNK; and EpRE-driven reporters along with a JNK-specific inhibitor peptide to determine the potential importance of c-Jun phosphorylation. Human bronchial epithelial (HBE1) and human hepatoma (HepG2) cells were exposed to 4-hydroxy-2-nonenal (HNE), and differences in the regulation of the same EpRE sequences were examined. We found that binding of c-Jun to EpRE sequences increased subsequent to HNE exposure in HepG2 cells; however, in HNE-exposed HBE1 cells, the binding of only phosphorylated c-Jun to the three EpRE sequences increased. Despite the increase in binding of phosphorylated c-Jun, reporter assays for EpRE's showed that inhibition of c-Jun phosphorylation had variable effects on basal and HNE-induced transcription of GCLC and GCLM in HBE1 cells. Thus, in terms of its role in mediating HNE induction of EpRE-mediated transcription, c-Jun seems to be a partner of Nrf2 and, whereas its phosphorylated form may predominate in one cell type versus another, the effects of phosphorylation of c-Jun on transcription can vary with the gene. This contrasts markedly with the well-established requirement for phosphorylation of c-Jun in the activation of AP-1/TRE-mediated transcription.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.