ArticleDevelopmental biology2009
MAP kinase dependent cyclinE/cdk2 activity promotes DNA replication in early sea urchin embryos.
Article in Developmental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- miR-92a-3p Promoted EMTFrontiers in cell and developmental biology · 2021Article
- Trapping, tagging and tracking: Tools for the study of proteins during early development of the sea urchin.Methods in cell biology · 2019Review
- Small interfering RNA against ERK1/2 attenuates cigarette smoke-induced pulmonary vascular remodeling.Experimental and therapeutic medicine · 2017Article
- Effects and underlying mechanisms of irisin on the proliferation and apoptosis of pancreatic β cells.PloS one · 2017Article
- ANGPTL3 is a novel biomarker as it activates ERK/MAPK pathway in oral cancer.Cancer medicine · 2015Article
- Article
- Integrins on eggs: focal adhesion kinase is activated at fertilization, forms a complex with integrins, and is necessary for cortex formation and cell cycle initiation.Molecular biology of the cell · 2013Article
- CREG promotes the proliferation of human umbilical vein endothelial cells through the ERK/cyclin E signaling pathway.International journal of molecular sciences · 2013Article
- ERK1/2 promotes cigarette smoke-induced rat pulmonary artery smooth muscle cells proliferation and pulmonary vascular remodeling via up-regulating cycline1 expression.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2013Article
- 9-Cis-retinoic acid induces growth inhibition in retinoid-sensitive breast cancer and sea urchin embryonic cells via retinoid X receptor α and replication factor C3.Molecular endocrinology (Baltimore, Md.) · 2012Article
- MAPK phosphorylation of connexin 43 promotes binding of cyclin E and smooth muscle cell proliferation.Circulation research · 2012Article
- Annexin A10 in human oral cancer: biomarker for tumoral growth via G1/S transition by targeting MAPK signaling pathways.PloS one · 2012Article
- Dynamic interactions of high Cdt1 and geminin levels regulate S phase in early Xenopus embryos.Development (Cambridge, England) · 2012Article
- Initiation of DNA replication after fertilization is regulated by p90Rsk at pre-RC/pre-IC transition in starfish eggs.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Sea urchins provide an excellent model for studying cell cycle control mechanisms governing DNA replication in vivo. Fertilization and cell cycle progression are tightly coordinated by Ca(2+) signals, but the mechanisms underlying the onset of DNA replication after fertilization remain less clear. In this study we demonstrate that calcium-dependent activation of ERK1 promotes accumulation of cyclinE/cdk2 into the male and female pronucleus and entry into first S-phase. We show that cdk2 activity rises quickly after fertilization to a maximum at 4 min, corresponding in timing to the early ERK1 activity peak. Abolishing MAP kinase activity after fertilization with MEK inhibitor, U0126, substantially reduces the early peak of cdk2 activity and prevents cyclinE and cdk2 accumulation in both sperm pronucleus and zygote nucleus in vivo. Both p27(kip1) and roscovitine, cdk2 inhibitors, prevented DNA replication suggesting cdk2 involvement in this process in sea urchin. Inhibition of cdk2 activity using p27(kip1) had no effect on the phosphorylation of MBP by ERK, but completely abolished phosphorylation of retinoblastoma protein, a cdk2 substrate, indicating that cdk2 activity is downstream of ERK1 activation. This pattern of regulation of DNA synthesis conforms to the pattern observed in mammalian somatic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.